2000
DOI: 10.1128/jvi.74.10.4831-4838.2000
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Rescue of Mumps Virus from cDNA

Abstract: A complete DNA copy of the genome of a Jeryl Lynn strain of mumps virus (15,384 nucleotides) was assembled from cDNA fragments such that an exact antigenome RNA could be generated following transcription by T7 RNA polymerase and cleavage by hepatitis delta virus ribozyme. The plasmid containing the genome sequence, together with support plasmids which express mumps virus NP, P, and L proteins under control of the T7 RNA polymerase promoter, were transfected into A549 cells previously infected with recombinant … Show more

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Cited by 62 publications
(52 citation statements)
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“…However, this approach requires a means to enable the generation of a mumps virus lacking SH. A reverse genetics system does exist for mumps virus (8). However, it is based on a vaccine strain, Jeryl Lynn (JL), that is severely attenuated in vivo and causes more CPE in infected cells than clinical isolates (35-38).…”
Section: Discussionmentioning
confidence: 99%
“…However, this approach requires a means to enable the generation of a mumps virus lacking SH. A reverse genetics system does exist for mumps virus (8). However, it is based on a vaccine strain, Jeryl Lynn (JL), that is severely attenuated in vivo and causes more CPE in infected cells than clinical isolates (35-38).…”
Section: Discussionmentioning
confidence: 99%
“…Construction of expression plasmids pMuVNP, pMuVP, and pMuVL (kindly provided by D. Clarke, Wyeth-Lederle Vaccines, Pearl River, NY) carrying the rJL N, P, and L genes, respectively, expression plasmids p-N, p-P, and p-L carrying the r88 N, P, and L genes, respectively, full-length cDNA clone pMuV(MPBS) carrying the "JL5" major component of the attenuated rJL vaccine, and full-length cDNA clone p88-1961 A271 encoding the neurovirulent r88 wild-type MuV strain are described elsewhere (10,21,23,24). Plasmids pMuV(MPBS) and p88-1961 A271 were renamed pJL and p88 A271 , respectively, in the present study for ease of readability.…”
Section: Methodsmentioning
confidence: 99%
“…The Huh 7.5 cells were a generous gift from Charles M. Rice. The recombinant mutant virus was constructed by using the Jeryl Lynn strain of mumps virus as previously described (8,28). The V protein E95D mutation was introduced by overlap extension PCR.…”
Section: Methodsmentioning
confidence: 99%