Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

Hoai, Bui Van; Nghiep, Ngo Dai; Quang, Dao An; Phuong, Nguyen Thi Nam

doi:10.46223/hcmcoujs.tech.en.8.1.339.2018

Cited by 1 publication

(1 citation statement)

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“…Research from [27] suggested that chitooligosaccharide powder with a molecular weight ranging from 5-10 kDa had an antioxidant activity of 17.61%. Je, et al, (2004) suggested that chitosan with a molecular weight of 1-5 kDa had the highest radical scavenging activity.…”

Section: Antioxidant Activity Of Cosmentioning

confidence: 99%

Antioxidant and Anticholesterol Activity of Chitooligosaccarides Hydrolyzed of Golden Apple Snail (Pomacea canaliculata) Shell Using Various Types of Enzyme

Rosida¹,

Jariyah²,

Larasati³

et al. 2023

Advances in Biological Sciences Research

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Chitosan (β1-4 N-acetyl D-glucosamine) is a chitin derivative compound which is obtained through the deacetylation stage, namely the process of releasing the acetamide group in chitin (CH3CONH) so that it becomes an amine group (NH2) in chitosan. The manufacture of chitosan is carried out through 3 stages, namely the process of deproteination, demineralization, and deacetylation. Chitosan has many functional properties because it has a reactive amine group (-NH2), some of its functional properties are antioxidant and anti-cholesterol. However, chitosan is a water insoluble compound, so its use is limited, especially in the food and medical fields. Therefore, chitosan needs to reduce its molecular weight by hydrolysis. Enzymatic hydrolysis is an alternative way that is safe and can be easily controlled. Completely Randomized Design (CRD) was used as the study design with two factors, namely the type of enzyme (cellulase, α-Amylase, and lipase) and concentration (0.1%; 0.5%; and 0.9%). The research showed that there were significant differences in the factors used on the molecular weight, enzymatic activity, antioxidant activity, and anticholesterolemic activity. The best treatment based on the results of this study was sample A1B3, namely chitooligosaccharide samples hydrolyzed using cellulase enzymes, with a concentration of 0.9%, a yield of 85.33% was obtained, with a molecular weight of 13.58 kDa, enzyme activity of 0.95 U/ml, antioxidant activity of 15.97%, and total cholesterol of 44.55 mg/dl (percentage of cholesterol reduction of 55.45%).

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Section: Antioxidant Activity Of Cosmentioning

confidence: 99%