Research Article Assessment of the rs4340 ACE gene polymorphism in acute coronary syndrome in a Western Mexican population.

Abstract

ABSTRACT. Acute coronary syndrome (ACS) is considered one of the main causes of death worldwide. Contradictory findings concerning the impact of the angiotensin-converting enzyme (ACE) gene on cardiovascular diseases have been reported. Previous conclusions point out that the variability in results depends on ethnicity and genetic polymorphisms to determine the association of rs4340 polymorphisms of the ACE gene and ACE circulating levels in ACS. Genotyping of rs4340 polymorphisms was performed in a total of 600 individuals from Western Mexico divided into two groups: the ACS and the control group (CG). The polymorphisms were identified by polymerase chain reaction. Serum ACE concentration was determined by enzyme-linked immunosorbent assay. D/D carriers had higher ACE levels than I/I carriers (3.6 vs 2.8 ng/mL, P < 0.0021) in the CG. The D/D genotype of the rs4340 polymorphism is associated with higher ACE concentration levels; however, the polymorphism was not associated with ACS.

The role of insertion-deletion polymorphism of the ACE gene in development of arterial hypertension in patients with chronic obstructive pulmonary disease

“…The known function of ACE is associated with the renin-angiotensin system, wherein ACE catalyzes the synthesis of angiotensin II vasoconstrictor from its non-vasoactive precursor, angiotensin I, and is also responsible for inactivation of vasodilator bradykinin 52 . Angiotensin II is a powerful vasopressor, which regulates blood pressure and fluid and electrolyte balance, chiefly through biosynthesis of aldosterone [53][54] . Association between I/D polymorphism of the ACE gene and the risk for COPD is a very timely and widely studied issue; however, existing data need to be clarified and appended [55][56][57] .…”

Genetic polymorphisms in the renin-angiotensin system and cognitive decline in Parkinson’s disease

“…To determine polymorphisms in the genes of angiotensinogen ( AGT : rs699, rs4762), AT1R ( AGTR1 : rs5186) and AT2R ( AGTR2 : rs5194, rs1403543) real-time PCR using pre-validated allelic discrimination TaqMan assays (rs699 assay ID: C_1985481_20, rs4762 assay ID: C_1985480_20, rs5186 assay ID: C_3187716_10, rs5194 assay ID: C_1841567_20, rs1403543 assay ID: C_7481825_10; Thermo Scientific, USA) was carried out. The insertion/deletion (I/D) polymorphism in the angiotensin-converting enzyme’s gene ( ACE ) was analysed by PCR with the second round of amplification for D homozygous carriers due to preferential amplification of D allele [ 23 ]. The genotypes of ACE I/D were determined by running the products in 3% agarose gel with ethidium bromide (1 μg/mL) for UV visualization.…”