Research Article CRISPR/Cas Class 2 systems and their applications in biotechnological processes

Mota, D.S.; Marques, Júlia; Guimarães, Jander Matos; Mariúba, L.A.M.

doi:10.4238/gmr18478

Cited by 11 publications

(7 citation statements)

References 52 publications

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“…16 The CRISPR/Cas systems are commonly divided into two classes, class I and class II, on the basis of structural variation of the Cas genes and their organization form. 17 The most regularly used is the type II CRISPR/Cas9 system, for gene editing, which makes use of a single Cas protein from Streptococcus pyogenes (SpCas9) to target specific DNA sequences. 18 CRISPR/Cas9 gene editing, called as "genetic scissors", has now revolutionized the clinical perspective for targeted interventions across the spectrum of varied genetic disorders.…”

Section: What Is Crispr/cas9 Systemmentioning

confidence: 99%

CRISPR/Cas9-mediated genome editing: from basic research to gene therapy

Goel

2024

Int J Res Med Sci

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CRISPR/Cas9 mediated genome editing is one of the most significant molecular tools discovered to edit the desired genes. It has ushered in a new era of novel possibilities of gene therapy. CRISPR/Cas9 system was originally observed as a part of the adaptive immune system in bacteria. It later on was adapted to carry precise and targeted alterations to the DNA in human cells to be used for gene therapy to correct genetic disorders and treat various severe diseases associated with the genetic changes. Besides this, the CRISPR/Cas9 system has been employed in pharmacogenomics to develop new drugs based on the patient’s genes, in modifying the organisms for research and even for diagnostic purposes in developing CRISPR based COVID-9 test. The recent approval of a CRISPR/Cas9 cellular gene therapy by FDA named “Casgevy” to treat sickle cell anemia is a testimonial to the potentials of CRISPR/Cas9 system in developing innovative gene therapies. This review details the mechanisms of CRISPR/Cas9 gene editing and its utilization in the ongoing clinical trials in the treatment of not only the monogenic disorders like sickle cell disease, thalassemia, and genetic blindness but also in treating multi-factorial diseases like cancers, cardiac diseases, diabetes, autoimmune diseases, viral infections such as human immunodeficiency virus (HIV) etc. An attempt has also been made to discuss the various limitations, challenges and ethical frameworks encompassing CRISPR/Cas9 based gene therapy in clinical settings.

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Section: What Is Crispr/cas9 Systemmentioning

confidence: 99%

CRISPR/Cas9-mediated genome editing: from basic research to gene therapy

Goel

2024

Int J Res Med Sci

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“…Recent developments have resulted in new methods to detect nucleic acids and infectious agents based on CRISPR systems linked mainly to Cas9, Cas12, and Cas13 enzymes. [ 42 , 111 ] COVID‐19 can be managed with the design of a therapeutic drug based on a similar approach as the CRISPR–Cas13 system, which aims at the SARS‐CoV‐2 ssRNA genome. A variety of methods based on the CRISPR–Cas13 system have been tested and proposed by researchers to counteract continuously mutating variants of the virus.…”

Section: Conclusion and Future Prospectivementioning

confidence: 99%

CRISPR‐based point‐of‐care diagnostics incorporating Cas9, Cas12, and Cas13 enzymes advanced for SARS‐CoV‐2 detection

Verma

Roychowdhury

Sahu

et al. 2022

J Biochem & Molecular Tox

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An outbreak of the novel beta coronavirus severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) first came to light in December 2019, which has unfolded rapidly and turned out to be a global pandemic. Early prognosis of viral contamination involves speedy intervention, disorder control, and good‐sized management of the spread of disease. Reverse transcription‐polymerase chain reaction, considered the gold standard test for detecting nucleic acids and pathogen diagnosis, provides high sensitivity and specificity. However, reliance on high‐priced equipped kits, associated reagents, and skilled personnel slow down sickness detection. Lately, the improvement of clustered regularly interspaced short palindromic repeat (CRISPR)‐Cas (CRISPR‐associated protein)‐based diagnostic systems has reshaped molecular diagnosis due to their low cost, simplicity, speed, efficiency, high sensitivity, specificity, and versatility, which is vital for accomplishing point‐of‐care diagnostics. We reviewed and summarized CRISPR–Cas‐based point‐of‐care diagnostic strategies and research in these paintings while highlighting their characteristics and challenges for identifying SARS‐CoV‐2.

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“…In a study by Anzalone et al, the prime editing method associated with the Cas9 protein and reverse transcriptase can describe the ingenious path for a strand of DNA and improve the efficiency of CRISPR-based methods. Even though this editing approach has not been developed for the SARS-CoV-2 virus, it has the potential to improve the editing process of DNA templates [88,89]. Other disadvantages are related to mismatch between RNA and target nucleotides [88].…”

Section: Crispr-based Nucleic Acid Detectionmentioning

confidence: 99%

Development of Diagnostic Tests for Detection of SARS-CoV-2

et al. 2020

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One of the most effective ways to prevent the spread of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is to develop accurate and rapid diagnostic tests. There are a number of molecular, serological, and imaging methods that are used to diagnose this infection in hospitals and clinical settings. The purpose of this review paper is to present the available approaches for detecting SARS-CoV-2 and address the advantages and limitations of each detection method. This work includes studies from recent literature publications along with information from the manufacturer’s manuals of commercially available SARS-CoV-2 diagnostic products. Furthermore, supplementary information from the Food & Drug Administration (FDA), Centers for Disease Control and Prevention (CDC), and World Health Organization (WHO) is cited. The viral components targeted for virus detection, the principles of each diagnostic technique, and the detection efficiency of each approach are discussed. The potential of using diagnostic tests that were originally developed for previous epidemic viruses is also presented.

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Research Article CRISPR/Cas Class 2 systems and their applications in biotechnological processes

Abstract: D.S. Mota et al. 2 them were selected for this review because they match with the keywords and the years of publication.

Cited by 11 publications

References 52 publications

CRISPR/Cas9-mediated genome editing: from basic research to gene therapy

CRISPR/Cas9-mediated genome editing: from basic research to gene therapy

CRISPR‐based point‐of‐care diagnostics incorporating Cas9, Cas12, and Cas13 enzymes advanced for SARS‐CoV‐2 detection

Development of Diagnostic Tests for Detection of SARS-CoV-2

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