Research Article Expression of miR-15b, miR-29b, miR-219 and miR-222 microRNAs in rats with focal cerebral ischemia submitted to physical exercise

Cirino, Mucio Luiz Assis; Porsani, Lucas Barbosa; Neto, Fermino Sanches Lizarte; Tazima, M.F.G.S.; Zimak, R.F.; Carlotti, Carlos Gilberto; Colli, Benedicto Oscar; Tirapelli, Luís Fernando; Tirapelli, Daniela Pretti Cunha

doi:10.4238/gmr18213

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…miR-205-5p, which was found to be activated in this study, suppresses the proliferation of pulmonary VSMCs [ 70 ], whereas miR-153, which was found to be inhibited in this study, attenuates hypoxia-induced excessive proliferation and migration of pulmonary arterial SMCs [ 71 ]. miR-22, which was found to be inhibited in this study, regulates VSMCs apoptosis (through p38MAPKα) and vascular remodeling in aortic dissection [ 72 ], whereas miR-219, which was found to be inhibited in this study, is associated with vascular ischemia during cerebral artery occlusion [ 73 ] ( Table 1 and Table 2 ). The expression of these miRNAs in this study and their relationship with vascular pathologies suggest that these miRNAs may be potential therapeutic targets in AVF.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional and Epigenetic Factors Associated with Early Thrombosis of Femoral Artery Involved in Arteriovenous Fistula

Rai

Agrawal

2022

Proteomes

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Arteriovenous fistulas (AVFs), created for hemodialysis in end-stage renal disease patients, mature through the outward remodeling of the outflow vein. However, early thrombosis and chronic inflammation are detrimental to the process of AVF maturation and precipitate AVF maturation failure. For the successful remodeling of the outflow vein, blood flow through the fistula is essential, but early arterial thrombosis attenuates this blood flow, and the vessels become thrombosed and stenosed, leading to AVF failure. The altered expression of various proteins involved in maintaining vessel patency or thrombosis is regulated by genes of which the expression is regulated by transcription factors and microRNAs. In this study, using thrombosed and stenosed arteries following AVF creation, we delineated transcription factors and microRNAs associated with differentially expressed genes in bulk RNA sequencing data using upstream and causal network analysis. We observed changes in many transcription factors and microRNAs that are involved in angiogenesis; vascular smooth muscle cell proliferation, migration, and phenotypic changes; endothelial cell function; hypoxia; oxidative stress; vessel remodeling; immune responses; and inflammation. These factors and microRNAs play a critical role in the underlying molecular mechanisms in AVF maturation. We also observed epigenetic factors involved in gene regulation associated with these molecular mechanisms. The results of this study indicate the importance of investigating the transcriptional and epigenetic regulation of AVF maturation and maturation failure and targeting factors precipitating early thrombosis and stenosis.

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Section: Discussionmentioning

confidence: 99%

Transcriptional and Epigenetic Factors Associated with Early Thrombosis of Femoral Artery Involved in Arteriovenous Fistula

Rai

Agrawal

2022

Proteomes

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“…They can simultaneously regulate targets that participate in the physiological processes of cerebral ischemia and are considered not only promising therapeutic agents in stroke, but also as potential biomarkers of the pathophysiological processes of cerebral ischemia (Liu et al, 2019). Recently, some studies have highlighted microRNAs as potential brain biomarkers that are involved in molecular mechanisms such as apoptosis, synaptic plasticity, proliferation, memory acquisition and loss, and differentiation (Cirino et al, 2019). Experiments with mice submitted to physical exercise after traumatic brain injury, performed by Bao et al (2014), obtained as results 32 microRNAs differentially expressed, of which 12 were hypoexpressed, and 20 were overexpressed.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-27b Expression in Rats Submitted to Physical Exercise and Experimental Cerebral Ischemia

et al. 2021

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Cerebral ischemia has not only a high mortality rate, which is the second leading cause of death worldwide, but is also responsible for severe disabilities in working age individuals, generating enormous public expending for treatment and rehabilitation of the affected individuals. The role of microRNAs in the pathophysiology of cerebral ischemia has been highlighted in current investigations. In addition, recent studies have also highlighted physical exercise as a possible protective factor both in the prevention and in the effects of cerebral ischemia, placing it as an important study resource. Thus, we investigated the role of physical exercise in experimental cerebral ischemia associated with the expression of microRNA-27b. 16 animals were used, divided into four experimental groups: Control, Physical Exercise, Cerebral Ischemia and Cerebral Ischemia associated with Physical Exercise. The real-time PCR methodology was used to analyze the expression of microRNA-27b. Although there were no statistically significant differences in the expression of microRNA-27b between the groups studied, the increased expression of microRNA-27b in the Physical Exercise group indicates its neuroprotective role in the pathophysiology of cerebral ischemia.

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“…The practice of regular physical exercise can improve quality of life and has been used as a mechanism of neuroprotection. The integration of brain neuronal impulses allows plastic changes that benefit the brain structures that have been compromised due to ischemia injury (Garbuzova-Davis et al, 2013;Cirino et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Research Article Lack of effect of exercise on the expression profiles of mir-16, mir-21, mir-155, caspase3 and bcl2 in a rat model of focal cerebral ischemia

Porsani¹,

Cirino²,

Neto³

et al. 2020

Genet. Mol. Res.

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Stroke is a major public health problem, with high mortality rates, and a high frequency of disability. Between 1990 and 2010, stroke increased from the fifth to the third leading cause of disability. In addition, its incidence has increased among younger people, with severe health consequences and increased social costs. There is evidence that physical exercise promotes neuroprotective effects when used as a therapeutic treatment. However, the mechanisms of neuroprotection are not yet well known. The objective of the study was to evaluate the expression profile of microRNAs miR-16, miR-21 and miR-155 and the CASPASE-3 and Bcl-2 genes previously related to apoptosis in the tissue (ischemic focus). Forty-eight Wistar rats were divided into four experimental groups: control group, ischemia group, ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 19 (4): gmr18594 L.B. Porsani et al. 2 exercise group and exercise + ischemia group. Before the ischemic procedure, the animals in the exercise and exercise + ischemia groups were submitted to a treadmill training protocol for four weeks. The training lasted 30 min a day at a speed of 18 m / min. For real-time PCR analysis, a fragment of the ischemic area was collected from each animal using a punch to analyze the expression of miRNAs; miR16, miR-21, miR-155, CASPASE-3 and Bcl-2 genes. In the animals that had physical exercise, there appeared to be a neuroprotective effect by the action of microRNAs and CASPASE-3, although no significant difference was observed. Further studies are needed to elucidate the role of apoptosis mechanisms in cerebral ischemia associated with physical exercise, as well as the role of microRNAs in the modulation of targets associated with this mechanism.

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Research Article Expression of miR-15b, miR-29b, miR-219 and miR-222 microRNAs in rats with focal cerebral ischemia submitted to physical exercise

Cited by 3 publications

References 25 publications

Transcriptional and Epigenetic Factors Associated with Early Thrombosis of Femoral Artery Involved in Arteriovenous Fistula

Transcriptional and Epigenetic Factors Associated with Early Thrombosis of Femoral Artery Involved in Arteriovenous Fistula

MicroRNA-27b Expression in Rats Submitted to Physical Exercise and Experimental Cerebral Ischemia

Research Article Lack of effect of exercise on the expression profiles of mir-16, mir-21, mir-155, caspase3 and bcl2 in a rat model of focal cerebral ischemia

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