Research Article Identification of bacterial leaf blight resistance genes in Malaysian local rice varieties

Hasan, Najmul; Rafii, M. Y.; Harun, Abdul Rahim; Ahmad, Fatin Nurliyana; Ismail, Nik Zainal Abidin Nik

doi:10.4238/gmr18545

Cited by 5 publications

(8 citation statements)

References 15 publications

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“…Similar to our finding, 34.29% germplasm were found to carry xa5 gene in India [14], 17.5% in Pakistan [53] and 29.62% in Nepal [16]. Alternatively, [15,17] Xoo [60]. However, in our study we identified that about 44.59% germplasm (33 out of 74) possessed resistant xa13 allele.…”

Section: Discussionsupporting

confidence: 89%

“…However, Xa21 had been identified as an effective and durable R gene against Xoo strains in Bangladesh [21,66], India [67,68], Vietnam [69], as well as in Korea [70]. Despite of its high effectiveness, Xa21 appeared to be a rare gene in terms of presence in the germplasm [12,14,16,17,53]. In our study we found only one germplasm carrying Xa21 gene.…”

Section: Discussionmentioning

confidence: 50%

“…However, in our study we identified that about 44.59% germplasm (33 out of 74) possessed resistant xa13 allele. Regrettably, in some previous researches led by [14,15,17] were not able to detect any xa13 gene in the germplasm derived from India, Thailand and Malaysia, respectively.…”

Section: Discussionmentioning

confidence: 86%

“…Pathogenicity test exposed variant bacterial blight disease reaction among studied germplasm which may be an indication of the presence of divergent genes/alleles in the germplasm collection. Nonetheless, vast majority of the germplasm were found moderately susceptible to highly susceptible with only a single highly resistant germplasm, which was a clear indication of high virulent status of the Xoo strains under the current study [14–17,47].…”

Section: Discussionmentioning

confidence: 90%

“…All these cloned R genes and some of the un-cloned R genes, like Xa7, are widely utilized in rice breeding programs especially focused on pyramiding of multiple resistant genes to develop bacterial blight resistant rice varieties. However, wild rice species and germplasm collections are widely considered as a rich reservoir of valuable genes and so, exploring germplasm collection in search of resistant cultivar has become a major focus of the researchers [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

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Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Anik¹,

Nihad²,

Hasan³

et al. 2021

Preprint

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Bacterial blight, one of the oldest and severe diseases of rice poses a major threat towards global rice production and food security. Thereafter, sustainable management of this disease has given paramount importance globally. In the current study, we explored 792 landraces to evaluate their disease reaction status against three highly virulent strains ( BXo69 , BXo87 and BXo93 ) of Xanthomonas oryzae pv . oryzae ( Xoo ). Subsequently, we intended to identify the possible candidate resistant ( R ) genes responsible for the resistant reaction using six STS markers correspond to Xa4 , xa5 , Xa7 , xa13 , Xa21 and Xa23 genes and finally, we evaluated morphological and molecular diversity of the potential bacterial blight resistant germplasm using quantitative traits and ISSR markers. Based on pathogenicity test, a single germplasm was found as highly resistant while, 33 germplasm were resistant and 40 were moderately resistant. Further molecular study on these 74 germplasm divulged that 41 germplasm carried Xa4 gene, 15 carried xa5 gene, 62 carried Xa7 gene, 33 carried xa13 gene, and 19 carried Xa23 gene. Only a single germplasm consisted of Xa21 gene. Interestingly, we found a wide range of gene combinations ranged from 2 to 4 genes among the germplasm resistant to bacterial blight and G3 genotype (Acc. No. 4216; highly resistant) having Xa4 , Xa7 , xa13 , Xa21 and G43 genotype (Acc. No. 1523; resistant) having Xa4 , xa5 , xa13 and Xa23 gene combination being the most effective against all the Xoo strains. Nonetheless, UPGMA dendrogram and heatmap analysis based on quantitative traits identified two important clusters viz. cluster-III and cluster-VIII with multiple desired traits However, genetic similarity based on ISSR marker data pointed out 3 germplasm, namely, G20 (Acc. No. 4004; contained Xa4, Xa7, xa13 genes), G17 (Acc. No. 3981; contained Xa7, xa13 genes) and G6 (Acc. No. 991; contained Xa4, Xa7, xa13, Xa23 genes) having comparatively lower genetic similarity (0.30, 0.37 and 0.38, respectively) with elite variety BRRI dhan28. Notably, Mantle test of molecular and morphological data indicated that there was a positive correlation (r = 0.113) between them. The outcome of this study would enrich and diversify the rice gene pool and would be useful for the development of durable bacterial blight resistant varieties.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Anik¹,

Nihad²,

Hasan³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Exploring of bacterial blight resistance in landraces and mining of resistant gene(s) using molecular markers and pathogenicity approach

Anik

Nihad

Hasan

et al. 2022

Physiol Mol Biol Plants

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Status of the resistance of selected rice (Oryzasativa) varieties to bacterial blight strains in Senegal

DOSSOU,

Foncéka,

Tall

et al. 2023

Preprint

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Rice plays an important role in achieving and maintaining food and nutritional security in the world. However, its productivity is affected by various constraints, including biotic and abiotic stresses, and several socio-economic constraints. Regarding biotic factors, bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major bacterial disease that causes severe yield losses of more than 70%, thereby threatening food and income security in most rice growing countries, including Senegal. Optimal control of this disease requires the use of host plant resistance, as it is economical and environmentally sustainable. Nevertheless, in the context of Senegal, the available sources of resistance are limited, and the potential for resistance among Senegalese rice varieties has been inadequately investigated. In this study we evaluated local rice germplasms for resistance to bacterial blight in Senegal. A trial was conducted in glasshouse conditions using a split-plot experimental design with 32 rice varieties as the main factor and five strains of Xanthomonas oryzae pv. oryzae (Xoo) as the secondary factor. Seven (07) of the popular rice varieties, including two (02) lowland (TOX 728-1 and BG 90 − 2), four (04) irrigated (ISRIZ 04, ISRIZ 05, ISRIZ 14 and SAHEL 202) and the variety GIGANTE exhibited full resistance against all strains tested. These varieties are suggested to be grown in Xoo-prone areas, and can be used for improving the resistance of other commercial varieties in other regions where Xoo is prevalent. A significant interaction between strains and varieties was detected, suggesting that pathogen race diversity may contribute to the determination of rice resistance to Xoo strains in Senegal. Among all bacterial strains, S82-4-1 of the race S4 exhibited the highest virulence. This strain can be used as reference for screening rice varieties in West-Africa.

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Research Article Identification of bacterial leaf blight resistance genes in Malaysian local rice varieties

Abstract: ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 19 (3): gmr18545 N.A. Hasan et al. 2 MXO 1410 isolates. These cultivars have potential as genetic materials for rice quality breeding programs.

Cited by 5 publications

References 15 publications

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Exploring of bacterial blight resistance in landraces and mining of resistant gene(s) using molecular markers and pathogenicity approach

Status of the resistance of selected rice (Oryzasativa) varieties to bacterial blight strains in Senegal

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