Research Article Selection of resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening

Abstract

The main goal of this work was to select resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. Fifty-five common bean genotypes, including differential varieties, characterized resistance sources, elite lines, cultivars and controls, were evaluated in a field inoculation trial and screened with SCAR markers linked to resistance genes that are important in Brazil. The field trial was carried out in Santo Antônio de Goiás, GO, Brazil, during the fall/winter growing season of 2014, using artificial inoculation with a mixture of six races of Colletotrichum lindemuthianum, selected based on their high virulence and prevalence in Brazil. Amplification reactions with the SCAR markers previously identified as linked to important anthracnose resistance genes on Brazil followed standard procedures. Twenty-eight of the 58 genotypes were resistant to anthracnose (mean severity score ≤ 3.5). Ten of these 28 resistant genotypes stood out because they presented a mean anthracnose severity score of 1.0. Four of the six SCAR markers tested shown to be useful for the assisted selection of their respective target genes (SH18 and SAS13 for Co-4 2 , SAB03 for Co-5, and SAZ20 for Co-6). Two carioca seeded elite lines were highlighted by the phenotypic and molecular ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 17 (3): gmr18066 A.F. Vieira et al 2 screening: K10 (Co-3 4 , Co-4 2 , Co-5 and Co-6) and K13 (Co-4²). The phenotypic and molecular characterization of candidate resistance sources to common bean anthracnose based on their disease reaction in field inoculation trials and on the analysis with molecular markers linked to resistance genes has shown to be a useful strategy. These results aid in the selection of donor parents and resistant lines to be preferably explored by common bean breeding programs in Brazil.

Response of French bean genotypes to Colletotrichum lindemuthianum and evaluation of their resistance using SCAR markers

“…3 ) and G2333 (Co-4 2 ) No 8 and12 respectively. This is in agreement with Vieira et al (2018) who did similar work in Brazil. The marker however, did not detect the possible gene for resistance among the French bean genotypes used in this study.…”

“…Race 65 may be considered as widespread as it was identified in Kakamega and Mwea. Races 65 and 73 have also been documented to be prevalent in Brazil (Vieira et al, 2018). Furthermore, race zero (0) was also identified in the present study as it could not infect any of the twelve anthracnose differential cultivars as previously reported in Kenya (Mogita et al, 2011b) and Uganda (Nkalubo, 2006).…”

Genetic mapping of the Andean anthracnose resistance gene present in the common bean cultivar BRSMG Realce

“…For this reason, anthracnose resistance in BRSMG Realce should be considered as a major gene or complex gene locus for breeding. It was also verified that Co-Realce segregates independently from Co-3 ( Table 1 ), the physically closest anthracnose resistance locus on Pv04 that has already been used by the Embrapa common bean breeding program to develop elite germplasm ( Vieira et al., 2018 ). Still considering physical map evidences, the positions of Co-3 (1,286,490 bp) ( Murube et al., 2019 ), Co-15 (9,432,376 bp) ( Sousa et al., 2015 ) and Co-16 (1,537,169 bp) ( Coimbra-Gonçalves et al., 2016 ) on Pv04 shown that those anthracnose resistance loci are distant from Co-Realce by 780,839 bp, 8,926,725 bp and 1,031,518 bp, respectively ( Figure 3 ).…”

Molecular Characterization of Parental Lines and Validation of Snp Markers for Disease Resistance in Common Bean

“…In the present study, four SNP markers associated with the anthracnose-resistant locus were analyzed. In case of the markers snpPV0068 (G:C) and snpPV0070 (G:T), the "G" alleles (of both SNPs) were identi ed in the G-2333, SEL 1308, PI 207262, K-10, K-13, and CNFC 5547 lines (Table S6), which are known sources of the resistant allele of Co-4 2 (Kelly and Vallejo 2004;Vieira et al 2018). Another 22 black grain lines also displayed this same allele (Table S6).…”

“…Portilla et al (2021) when evaluating the Co-4 marker snpPV0069 (closely linked to the snpPV0068 and snpPV0070), developed from G2333 genotype (Lobaton et al 2018) and available at HTPG portfolio, did not identify any signi cant effect of association, which was suggestive of a race speci c resistance gene interaction. The Co-4 2 is a dominant allele of the Co-4 locus and has been used by breeding programs worldwide (Kelly and Vallejo 2004;Vieira et al 2018) due to its broad spectrum of resistance to several C. lindemuthianum pathotypes (Balardin and Kelly 1998;Silvério et al 2002).…”