Research Article Transferability of heterologous microsatellite loci between species of Euterpe genus

AzÃavedo, H.S.F.S; Benvindo, F.D; Cavalcante, Lourianne Nascimento; Haverroth, Moacir; Wadt, L. H. de O.; Campos, Tatiana de

doi:10.4238/gmr16039825

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…Loci EE41 and EE47 had the lowest annealing temperature (45.8 °C), and locus EE45 had the highest annealing temperature (57.3 °C). The results also confirmed the transferability of loci from E. edulis to E. precatoria (AZÊVEDO et al, 2017). These results indicated that DNA extracted from leaflets of E. precatoria did not contain contaminants such as polysaccharides and secondary metabolites, which could affect DNA quality (SIKA et al, 2015).…”

Section: Resultssupporting

confidence: 70%

Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA

et al. 2019

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Biomedicina -Faculdade Meta -FAMETA, Rio Branco, AC, Brasil.; 3. Professor, Doutor, Instituto Federal do Acre -IFAC, Cruzeiro do Sul, AC, Brasil.; 4. Analista da Empresa Brasileira de Pesquisa Agropecuária -Embrapa Acre, Rio Branco, AC, Brazil.; 5. Pesquisadora, Doutora, Empresa Brasileira de Pesquisa Agropecuária -Embrapa Rondônia, Porto Velho, RO, Brasil.; 6. Pesquisadora, Doutora, Empresa Brasileira de Pesquisa Agropecuária -Embrapa Acre, Rio Branco, AC, Brasil, tatiana.campos@embrapa.br. ABSTRACT:The objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/µL (7 days in transport buffer) to 702.00 ng/µL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/µL (30 days in silica gel) to 2,850.00 ng/µL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in leaf tissue preservation and DNA concentration, all treatments were effective for DNA isolation and it was possible to amplify genomic regions of microsatellite markers by PCR. It was concluded that leaflets of E. precatoria stored in a lyophiliser and processed with an automatic macerator resulted in satisfactory DNA for molecular studies.

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Section: Resultssupporting

confidence: 70%

Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA

et al. 2019

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“…The transferability of microsatellite markers depends on the conservation of the primer binding sites (Fagundes et al 2016). The rather high success in cross-transferability of markers tested in this study suggested close genetic relationships within and between Aquilaria and Gyrinops.…”

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confidence: 99%

Cross-amplification of microsatellite markers across agarwood-producing species of the Aquilarieae tribe (Thymelaeaceae)

Pern

Lee

et al. 2020

3 Biotech

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Tree species in the Aquilarieae tribe of the Thymelaeaceae family produce agarwood, a natural product highly valued for its fragrance, but the species are under threat due to indiscriminate harvesting. For conservation of these species, molecular techniques such as DNA profiling have been used. In this study, we assessed cross-amplification of microsatellite markers, initially developed for three Aquilaria species (A. crassna, A. malaccensis, and A. sinensis), on ten other agarwood-producing species, including members of Aquilaria (A. beccariana, A. hirta, A. microcarpa, A. rostrata, A. rugosa, A. subintegra, and A. yunnanensis) and Gyrinops (G. caudata, G. versteegii, and G. walla), both from the Aquilarieae tribe. Primers for 18 out of the 30 microsatellite markers successfully amplified bands of expected sizes in 1 sample each of at least 10 species. These were further used to genotype 74 individuals representing all the 13 studied species, yielding 13 cross-amplifiable markers, of which only 1 being polymorphic across all species. At each locus, the number of alleles ranged from 7 to 23, indicating a rather high variability. Four markers had relatively high species discrimination power. Our results demonstrated that genetic fingerprinting can be an effective tool in helping to manage agarwood genetic resources by potentially supporting the chainof-custody of agarwood and its products in the market.

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“…The illegal logging of adult individuals of E. precatoria to remove the heart of palm and wood, causes a reduction in the number of individuals, thus, alleles of interest are lost and not transmitted to the next generations (Azêvedo et al, 2017). Knowing the distribution of genetic diversity among and within natural populations is very important for the development of conservation strategies.…”

Section: Genéticamentioning

confidence: 99%

Diversity and genetic structure of natural populations of the palm tree Euterpe precatoria (Arecaceae)

Cruz¹,

Dardengo

Rossi

et al. 2022

Rev. Biol. Trop.

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Introduction: The natural ecosystems of northern Mato Grosso, Brazil, are in process of fragmentation, mainly due to population growth and the expansion of agriculture. This endangers the palm Euterpe precatoria (locally known as açaí), used for construction, palm hearts, juices and ice cream. Objective: To evaluate the local diversity and genetic structure in native populations of E. precatoria. Methods: We collected leaves from 106 fruiting palms from five populations in Mato Grosso State, for analysis of microsatellite markers with Polymerase Chain Reaction. Results: The five SSR loci revealed a total of 30 alleles, ranging from 5 (EE23 and EE43) to 7 (EE2 and EE15), with an average of 6 alleles per locus. The mean PIC was 0.74 and confirmed low heterozygosity and inbreeding. The UPGMA dendrogram produced two groups and molecular variance revealed greater genetic differentiation within populations. The high levels of homozygous microsatellite loci indicate low genetic diversity. Conclusions: These populations have low gene diversity, high average number of alleles per locus, and rare and exclusive alleles. We recommend the establishment of permanent conservation units with corridors among them.

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Research Article Transferability of heterologous microsatellite loci between species of Euterpe genus

Cited by 7 publications

References 16 publications

Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA

Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA

Cross-amplification of microsatellite markers across agarwood-producing species of the Aquilarieae tribe (Thymelaeaceae)

Diversity and genetic structure of natural populations of the palm tree Euterpe precatoria (Arecaceae)

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