The performance of a spindle will gradually decline, the vibration intensity will increase, and the temperature rise will become abnormal with the accumulation of service time. Consequently, the accuracy of the machining product will not meet its production requirements. Studies on the variation characteristics of the spindle unit have clarified the reasons for its abnormal vibration and temperature rise, in principle, to help enterprises conduct preventive maintenance before any serious failure occurs and improve production efficiency. Based on the Timoshenko beam model and rotor dynamics theory, this study uses the finite element method (FEM) to analyze the vibration characteristics of the spindle rotor system. Moreover, it thoroughly analyzes the influence of the spindle bearing heat on the stiffness of the spindle system and identifies the resonance conditions of the spindle rotor within the power frequency range. This research has identified that when the vibration frequency of the spindle operates at a speed of 12,000 r/min, if its vibration frequency is lower than 200 Hz, it will be affected by abnormal vibrations during startup, which will weaken the health status of the spindle and reduce its service life. Similarly, when the working speed of the spindle is 30,000 r/min, if its vibration frequency is lower than 50 Hz, abnormal vibration will occur during startup and operation, thereby reducing its service life.