Research letter: A new fluorescent three‐dimensional and deep‐imaging technique for histological identification of individual tumor cells in extramammary Paget's disease

Murakami, Masamoto; Kawakami, Rika; Niko, Yosuke; Tsuda, Tetsuo; Imamura, Takeshi

doi:10.1111/exd.14759

Experimental Dermatology

2023

DOI: 10.1111/exd.14759

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Research letter: A new fluorescent three‐dimensional and deep‐imaging technique for histological identification of individual tumor cells in extramammary Paget's disease

Masamoto Murakami

Rika Kawakami

Yosuke Niko

et al.

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2023

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Cited by 2 publications

References 7 publications

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Pyrene-fused dioxaborine-based merocyanines with high brightness, photostability, and fluorogenic function for deep-skin tissue imaging of a living mouse

Nishihara,

Watanabe,

Kawakami

et al. 2023

Bulletin of the Chemical Society of Japan

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Bright polymethine dyes, typified by carbocyanines, are employed in various fluorescence techniques such as the 3D visualization of living cell morphology and the tracking of extracellular vesicles in the blood vessels of a zebrafish. However, they often exhibit low photostability, particularly for dyes with red-shifted absorption/fluorescence wavelengths due to extended polymethine length, and limited photofunctionality. This limitation restricts their utility in specific applications requiring high-power excitation and/or a wash-free approach. This study introduces novel merocyanine dyes, MCPY3 and MCPY5, comprising a newly developed pyrene-fused dioxaborine and polymethine chain. Despite their minimal polymethine lengths, their absorption/fluorescence wavelengths reside in the red to near infra-red regions due to the substantial π-conjugation system of pyrene. Moreover, they exhibit a considerably superior photostability to carbocyanine dyes and fluorogenic behavior between low (ON) and high (OFF) polar solvents, while maintaining brightness comparable to carbocyanine. Leveraging these advantages, the hydrophilic analogs of MCPY3, MCPY3S, was applied to two-photon microscopy imaging of the skin tissues on the finger of a living mouse. The dye clearly visualized the individual cell morphology in the epidermis and the elastin within the dermis, highlighting the potential of the new dye as a valuable tool for fundamental dermatological and histological studies.

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Pyrene-fused dioxaborine-based merocyanines with high brightness, photostability, and fluorogenic function for deep-skin tissue imaging of a living mouse

Nishihara,

Watanabe,

Kawakami

et al. 2023

Bulletin of the Chemical Society of Japan

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Facile Preparation of Near Infrared-Luminescent Protein Complexes with Conjugated Polymers Consisting of Boron Azobenzene Units

Yoo

Nakamura

Kanjo

et al. 2023

Bulletin of the Chemical Society of Japan

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We report herein facile preparation of near infrared (NIR)-luminescent protein complexes with conjugated polymers. We have discovered that solid-state luminescence in the NIR region can be obtained from the series of conjugated polymers consisting of boron azobenzene complexes. We demonstrate in this paper that protein molecules can be modified through adsorption with the boron azobenzene-containing conjugated polymers simply by mixing in an aqueous buffer and subsequently purification with filtration followed by freeze drying. The modified protein complexes can exhibit NIR emission in the buffer and high dispersibility. In particular, comparing to the complex with indocyanine green (ICG), which is a conventional NIR-luminescent dye, polymer-modified protein complexes showed higher resistance to photobleaching. Finally, by using lipase as a scaffold, we confirmed that enzymatic activity can be detected after polymer modification.

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Research letter: A new fluorescent three‐dimensional and deep‐imaging technique for histological identification of individual tumor cells in extramammary Paget's disease

Cited by 2 publications

References 7 publications

Pyrene-fused dioxaborine-based merocyanines with high brightness, photostability, and fluorogenic function for deep-skin tissue imaging of a living mouse

Pyrene-fused dioxaborine-based merocyanines with high brightness, photostability, and fluorogenic function for deep-skin tissue imaging of a living mouse

Facile Preparation of Near Infrared-Luminescent Protein Complexes with Conjugated Polymers Consisting of Boron Azobenzene Units

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