Research Note: Evaluation of a heat stress model to induce gastrointestinal leakage in broiler chickens

Abstract

The purpose of this study was to evaluate heat stress as a model to induce gastrointestinal leakage in broiler chickens. On the day of hatch, 320 chicks were allocated into 8 environmental chambers, 4 thermoneutral ( TN ) and 4 continuous heat stress ( HS ). Each chamber was divided into 2 pens containing separate feeders and water jugs (8 replicates per treatment, 20 birds/pen). The environment was established to simulate production setting as best possible for the first 21 D. A gradual reduction of temperature from 32°C to 24°C with relative humidity at 55 ± 5% was adopted for the first 21 D. At the time of HS, the HS groups were exposed to 35°C from Day 21 to 42, while thermoneutral ones were maintained at 24°C from Day 21 to 42. Chickens were equipped with a Thermochron temperature logger for continuous monitoring of core body temperature. The environmental temperature and relative humidity were continuously recorded. Fluorescein isothiocyanate–dextran ( FITC-d ) was orally gavaged to 2 chickens/replicate ( n = 16) randomly selected on days 21, 28, 35, and 42. After 1 h of oral gavage, blood samples were collected to determine the passage of FITC-d. Tibias were removed from all chickens to evaluate break strength only on 21 D and 42 D (before HS and at the end of the trial). Performance parameters were evaluated weekly from 21 D to the end of the trial. Body temperature was significantly ( P < 0.05) increased after 2 h of starting HS and remained that way until the end of the study. Chronic HS caused an increase in core body temperature which decreased feed intake, body weight, and feed efficiency (28, 35, and 42 D) when compared with control TN chickens. Similarly, serum FITC-d was significantly increased in HS chickens at all points of evaluation. Chronic HS also caused a significant reduction of bone strength at 42 D when compared with the control chickens. The results from the present study suggest that HS can be a robust model to induce gut leakage in broiler chickens.

Evaluation of Curcumin and Copper Acetate Against Salmonella Typhimurium Infection, Intestinal Permeability, and Cecal Microbiota Composition in Broiler Chickens

“…Although the markers mentioned above were not determined in the present study, FITC-d, a large molecule (3-5 kDa) that, under normal intestinal health conditions, does not leak through the epithelium, was used. However, when there is damage to the epithelium, the permeability of FITC-d increases so that it can be quanti ed in serum [55]. In the present study, all treated groups showed lower serum FITC-d concentrations compared to the PC group (Table 2).…”

Evaluation of curcumin and copper acetate against Salmonella Typhimurium infection, intestinal permeability, and cecal microbiota composition in broiler chickens

“…However, when there is damage to the epithelium, the permeability of FITC-d increases so that it can be quanti ed in serum [55]. In the present study, all treated groups showed lower serum FITC-d concentrations compared to the PC group (Table 2).…”

Evaluation of curcumin and copper acetate against Salmonella Typhimurium infection, intestinal permeability, and cecal microbiota composition in broiler chickens

“…In the present study, FITC-d, a large molecule (3-5 kDa) that, under normal intestinal health conditions, does not leak through the epithelium, was used to assess intestinal permeability. However, when there is damage to the epithelium, the permeability of FITC-d increases so that it can be quanti ed in serum [57]. In the present study, all treated groups showed lower serum FITC-d concentrations compared to the PC group (Table 2).…”