Research Note: IsomiRs of chicken miR-146b-5p are activated upon Salmonella enterica serovar Enteritidis infection

Hu, Geng; Liu, Liying; Miao, Xiuxiu; Zhao, Yanan; Li, Xianyao

doi:10.1016/j.psj.2022.101977

Cited by 4 publications

(9 citation statements)

References 10 publications

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“…Enteritidis (OTU607) was detected in treatment groups (CT and RT) but not in the control groups (CC and RC; Supplementary Table S1 ), suggesting the success of challenge work. As described in our previous study ( Hu et al, 2022 ), after the merging, there were total 15,205 genes in RNA-seq dataset of 12 samples and 525 differentially expressed genes (|log 2 FC| > 1 and Padj < 0.05; Supplementary Table S2 ), of which 394 and 131 were up-and down-regulated compared to the control (MC), respectively. The RNA-seq data were validated with 29 genes by RT-qPCR.…”

Section: Resultsmentioning

confidence: 99%

“…As described previously ( Hu et al, 2022 ), the chicks used in experiment were two populations of reciprocal crosses [the Cross (Guangxi Yao ♂ × Jining Bairi ♀) and the Reverse-cross (Guangxi Yao ♀ × Jining Bairi ♂)], which derived from two Chinese local chickens, Guangxi Yao and Jining Bairi. For each crossbreed, 100 mixed gender, Salmonella -negative birds were randomly divided into two groups and housed in separated isolators (32 to 35°C, 50 to 60% RH, and 24 h light) with ad libitum access to water and antibiotic-free feed.…”

Section: Methodsmentioning

confidence: 99%

“…As described in our previous study ( Hu et al, 2022 ), to focus on the common characteristics across the two crossbreeds and increase the reliability of the analysis, we combined the experiments of reciprocal crosses. That is, the control groups (CC and RC) and the treatment groups (CT and RT) were merged into MC and MT, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The details were described in our previous study ( Hu et al, 2022 ). RNA sequencing and the preliminary analysis were completed in Shanghai OE Biotech Co., Ltd. (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…The result of 16S rRNA gene sequencing showed that S. Enteritidis (OTU607) was detected in treatment groups (CT and RT) but not in the control groups (CC and RC; Supplementary Table S1), suggesting the success of challenge work. As described in our previous study (Hu et al, 2022), after the merging, there were total 15,205 genes in RNA-seq…”

Section: The Triggered Inflammatory Response In Cecum Tissuementioning

confidence: 99%

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The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis

Liu

Miao

et al. 2022

Front. Microbiol.

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By combining the experiments of reciprocal crosses of chicken infected with Salmonella enterica serovar Enteritidis (S. Enteritidis), we focused on the common response of cecal microbiota to an inflammatory state in respect of transcriptome and microbiome. The inoculation of S. Enteritidis improved the microbial diversity and promoted the microbiota evolution in our infection model. Correlation analysis between bacteria and inflammation-related genes showed that some intestinal microorganisms were “inflammophile” and thrived in an inflamed environment. The global function of cecal microbiome was to maintain the homeostasis likely by the up-regulation of microbial metabolism pathway in bacitracin, putrescine, and flavonoids production, although the bacitracin may affect the symbiotic bacteria Enterococcus. The action of S. Enteritidis had close relationships with multiple inflammation-related genes, including the genes PTAFR, LY96, and ACOD1 which proteins are related to the binding and tolerance of LPS, and the genes IL-18, IL-18R1 and IL-18RAP which products can form a functional complex and transmit IL-18 pro-inflammatory signal. Additionally, the infection of S. Enteritidis aroused the transcription of EXFABP, which protein has a potential to sequestrate the siderophore and might cause the decline of Escherichia-Shigella and Enterococcus. S. Enteritidis can escape from the sequestrating through the salmochelin, another kind of siderophore which cannot be recognized by EXFABP. Probably by this way, S. Enteritidis competed with the symbiotic bacteria and edged out the niches. Our research can help to understand the interplay between host, pathogen, and symbiotic bacteria.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The details were described in our previous study ( Hu et al, 2022 ). RNA sequencing and the preliminary analysis were completed in Shanghai OE Biotech Co., Ltd. (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Section: The Triggered Inflammatory Response In Cecum Tissuementioning

confidence: 99%

See 3 more Smart Citations

The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis

Liu

Miao

et al. 2022

Front. Microbiol.

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Symbiotic bacteria stabilize the intestinal environment by producing phenylpropanoids

Liu

Miao

et al. 2022

Microbial Biotechnology

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Salmonella enterica serovar Enteritidis (S. Enteritidis) can colonize in the intestinal tract of chickens and transmit to humans. In order to decrypt the mechanism of avian resistance to S. Enteritidis, we utilized two China local chicken breeds to generate the reciprocal crosses (the Cross and the Reverse-cross). The two lines of hybrids were orally inoculated with S. Enteritidis at 2-day old and sampled at 3 days post-inoculation. Along the analysis direction of multiomics, differential metabolites, functional pathways and correlated microbes, we found that 12 species of microbes thrived upon S. Enteritidis challenge and probably contributed to the intestinal stability in the Cross by enhancing the production of phenylpropanoids. Our findings can help to understand the symbiotic and resistant mechanisms derived from the intestinal microbiota.

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In-depth transcriptome profiling of Cherry Valley duck lungs exposed to chronic heat stress

Liu,

Sun,

et al. 2024

Front. Vet. Sci.

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Amidst rising global temperatures, chronic heat stress (CHS) is increasingly problematic for the poultry industry. While mammalian CHS responses are well-studied, avian-specific research is lacking. This study uses in-depth transcriptome sequencing to evaluate the pulmonary response of Cherry Valley ducks to CHS at ambient temperatures of 20°C and a heat-stressed 29°C. We detailed the CHS-induced gene expression changes, encompassing mRNAs, lncRNAs, and miRNAs. Through protein–protein interaction network analysis, we identified central genes involved in the heat stress response—TLR7, IGF1, MAP3K1, CIITA, LCP2, PRKCB, and PLCB2. Subsequent functional enrichment analysis of the differentially expressed genes and RNA targets revealed significant engagement in immune responses and regulatory processes. KEGG pathway analysis underscored crucial immune pathways, specifically those related to intestinal IgA production and Toll-like receptor signaling, as well as Salmonella infection and calcium signaling pathways. Importantly, we determined six miRNAs—miR-146, miR-217, miR-29a-3p, miR-10926, miR-146b-5p, and miR-17-1-3p—as potential key regulators within the ceRNA network. These findings enhance our comprehension of the physiological adaptation of ducks to CHS and may provide a foundation for developing strategies to improve duck production under thermal stress.

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Research Note: IsomiRs of chicken miR-146b-5p are activated upon Salmonella enterica serovar Enteritidis infection

Cited by 4 publications

References 10 publications

The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis

The response of cecal microbiota to inflammatory state induced by Salmonella enterica serovar Enteritidis

Symbiotic bacteria stabilize the intestinal environment by producing phenylpropanoids

In-depth transcriptome profiling of Cherry Valley duck lungs exposed to chronic heat stress

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