Research Note: The Presence of Yersinia enterocolitica and Other Yersinia Species on the Carcasses of Market Broilers

Abstract

Sixty ready-to-cook broiler carcasses obtained from several local supermarkets were tested for the presence of Yersinia enterocolitica and other Yersinia species. In the present study, the authors used two enrichment broths, yeast-extract/rosebengal-bile oxalate sorbose (YER-BOS) and phosphate-buffered saline with a postenrichment KOH treatment (PBS-KOH), and two plating media, cefsulodin-irgasan-novobiocin (CIN) and pectin agar. Yersinia organisms were found on 34 of 60 carcasses (56.7%) and Y. enterocolitica, on 16 of 60 carcasses (26.7%). There was no significant difference between CIN and pectin agar; however, PBS-KOH yielded a significantly higher (P less than or equal to .05) detection rate than YER-BOS, regardless of the plating media used. In addition to Y. enterocolitica, Y. frederiksenii and Y. intermedia were also isolated from the market broilers. None of the Y. enterocolitica isolates were found to be presumptively virulent.

The use of a surface adhesion immunofluorescent (SAIF) method for the rapid detection ofYersinia enterocoliticaserotype O:3 in meat

“…It is one of a small number of pathogens capable of significant growth at refrigeration temperatures (Swaminathan et al 1982;Walker et al 1990). Pathogenic strains occur widely in food animals, particularly pigs, and have been isolated from both raw and cooked meats worldwide (Hanna et al 1976;Schiemann 1980;Cox et al 1990;De Boer and Nouws 1991;Logue et al 1996). As a consequence, effective methods for the rapid detection and/or enumeration of Y. enterocolitica are required.…”

“…Pathogenic strains occur widely in food animals, particularly pigs, and have been isolated from both raw and cooked meats worldwide (Hanna et al . 1976; Schiemann 1980; Cox et al . 1990; De Boer & Nouws 1991; Logue et al .…”

Monitoring of plasmid dissociation and pathogenic potential amongYersinia enterocolotica andYersinia pseudotuberculosis during storage of refrigerated pork meat

“…Evidently, the augmented background pressure leads to more active plasmid dissociation among the population of less pathogenic serotypes of Y. enterocolitica (O:9 and O:3) but still preserving their better survivability. Thus, both serotypes seem to have a better adaptive potential resembling other Y. enterocolitica strains in eggs (Ericson and Jenkins, 1992), mussel tissue (Hudson and Avery, 1994), broilers (Bok et al, 1986;Cox et al, 1990), water (Stern and Pierson, 1979), etc. In a different experimental design Goverde et al (1994) reported differences in the growth rates in the plasmid bearing and plasmid cured strains of serotype O:9 in pure culture.…”

Y. enterocolitica and Y. pseudotuberculosis

“…; Iushchenko et al, 1982;Pavlov, 1985;Ding et al, 1986;Schiemann, 1987;De Centorbi et al, 1989;Hamama et al, 1992;Durisin et al, 1997) A wide range of animals: domestic (cat and dog), stock farm (chinchilla, mink, pig, rabbit, cow, goose, horse, sheep, and buffalo), zoo (monkey), wild (raccoon, fox, snail, frog, beaver, deer, ocelot, crab, flies, and fleas), birds (robin), shellfish (oyster), and many species of small rodents (McClure et al, 1971;Wauters et al, 1971;Alonso and Bercovier, 1975;Toma and Deidrick, 1975;Wauters and Janssens, 1976;Kapperud, 1977;Servan et al, 1979;Iushchenko et al, 1982;Gurleva et al, 1985;Kato et al, 1985;Walker and Grimes, 1985;Shayegani et al, 1986;Weber et al, 1987;Cox et al, 1990;Escudero et al, 1991;Weynants et al, 1996;Zheng and Xie, 1996;Funk et al, 1998;Letellier et al, 1999;Naktin and Beavis, 1999) Most of the strains isolated from the environment belong to the nonpathogenic biotype 1A, with the exception of pathogenic strains of biotype 1B, which are frequently isolated from water.…”