Research of Indicators of Oxidative Stress in the Kidneys of Immature Rats With Hyperhomocysteinemia

Abstract

Purpose: Hyperhomocysteinemia in children can develop as a result of genetic defects, endocrine abnormalities or under the influence of dietary factors. An elevated level of homocysteine is considered a risk factor for the progression of chronic kidney disease. The aim of the work was to investigate the indicators of oxidative stress in the homogenate of the kidneys of immature rats in control and with hyperhomocysteinemia. Methods: The concentration of reduced and oxidized glutathione, the activity of superoxide dismutase, catalase and nitric oxide synthase were determined. The model of hyperhomocysteinemia was reproduced on one-month-old male rats, which were kept on a standard vivarium diet. The experimental group was intragastrically administered by D,L-thiolactone homocysteine hydrochloride in a 1% starch solution at a dose of 200 mg/kg of body weight 1 per day for 8 weeks. The corresponding volume of 1% starch solution was injected into the control group of animals. The activity of superoxide dismutase, catalase and nitric oxide synthase were determined spectrophotometrically. Concentration of reduced and oxidized glutathione by fluorometric method. Results: It was established that upon hyperhomocysteinemia the concentration of reduced glutathione, the activity of superoxide dismutase, catalase, and nitric oxide synthase was decreased against the background of an increase in the concentration of oxidized glutathione in the homogenate of the kidneys of immature rats. Conclusions: The obtained results indicate that in the kidneys of immature rats, the development of oxidative stress occurs in the direction characteristic of adult animals. The obtained results indicate that in the kidneys of immature rats the development of oxidative stress resembles the adult animals. The obtained results showed a decrease in the concentration of reduced glutathione and the activity of antioxidant defense enzymes which may indicate the development of pathological processes in the kidneys

Enzymatic and non-enzymatic link components of antioxidant defence in subcellular fractions of rat liver under the influence of diethyl phthalate

“…Superoxide dismutase activity in the cytosolic fraction of rat liver under the influence of diethyl phthalate Note (hereinafter): Control -intact animals (M±m, n=18); Group II -rats injected with diethyl phthalate at a dose of 2.5 mg per kg of body weight (M±m, n=18); Group III -rats injected with diethyl phthalate at a dose of 5.4 mg/kg of body weight (M±m, n=18); * -P <0.05; ** -P <0.01; *** P <0.001compared to the control group; # -statistically significant difference group IІI compared to group ІІ; & -statistically significant difference compared to 14 days within the same group When DEP was administered at a dose of 5.4 mg/kg of body weight, a 1.3-fold decrease in the enzymatic activity of SOD was observed compared to that of intact animals (Р ˂0.05) (Fig. 1 The decrease in the enzymatic activity of SOD is probably due to the accumulation of a large amount of H 2 O 2 , which is released during O 2 •dismutation and is an inhibitor that leads to the inactivation of this enzyme (Zheng et al, 2013;Serbin et al, 2022).…”