Research of Inonotus obliquus Oligosaccharide in Prevention of Hyperlipidemia

Abstract

In this study, hot water was used to extract Inonotus obliquus oligosaccharide. DEAE-cellulose and Sepharose G-200 were used to purify Inonotus obliquus oligosaccharide. Inonotus obliquus oligosaccharide IOP-2A was obtained. Its molecular weight Mw is about 1000 Da. The monosaccharide composition and molar ratio were glucose : xylose : galactose : mannose = 54.1 : 13.6 : 13.2 : 6.7. In addition, it also contains a small amount of galactose, gluconic acid, rhamnose, and fucose. IOP-2A contained mainly β-glycosidic bonds. Among them, 1,4-glycosidic bonds accounted for 9.2%, and 1,6-glycosidic bonds accounted for 85.1%. Oligosaccharide macromolecules formed a layered structure. Mouse experiments showed that IOP-2A had the function of preventing hyperlipidemia. At the same time, IOP-2A had a certain protective effect on the liver and kidney. The mechanism of IOP-2A in preventing hyperlipidemia was obtained from the perspective of mouse intestinal flora.

A review on the cultivation, bioactive compounds, health-promoting factors and clinical trials of medicinal mushrooms Taiwanofungus camphoratus, Inonotus obliquus and Tropicoporus linteus

“…Hypolipidemic effects were also observed in the 250 mg/kg and 500 mg/kg I. obliquus aqueous extract treatment groups, with decreased TC, TG, and LDL-C and increased liver glycogen and HDL-C in high-fat diet-inducedC57BL/6 mice via the regulation of the PI3K/Akt and AMPK/ACC signalling pathways [ 208 ]. Similar hypolipidemic effects were also reported in oligosaccharides of I. obliquus via modification of the intestinal bacteria in high-fat fed mice in which the ratio between the population of Firmicutes to Bacteroidetes faecal flora was reduced in treated groups, reducing the production of fatty acids and thus lowering blood lipids [ 209 ].…”

Therapeutic properties of Inonotus obliquus (Chaga mushroom): A review

“…Oligosaccharides isolated from I. obliquus also provided protection against hyperlipidaemia. Kunming mice were fed a high-fat diet (HFD) for 1 week, then allowed to eat and drink freely with I. obliquus oligosaccharide (IOP-2A) administered by gavage in designated groups (Wu et al 2021 ). Biochemical analysis of mice serum showed that IOP-2A is able to ameliorate dyslipidaemia by decreasing TC, TG, and LDL-C levels while increasing HDL-C concentrations.…”