Research of PDGF-BB Gel on the Wound Healing of Diabetic Rats and Its Pharmacodynamics

Li, Haihong; Fu, Xiaobing; Zhang, Lei; Huang, Qingjun; Wu, Zhigu; Sun, Tao

doi:10.1016/j.jss.2007.02.044

Cited by 71 publications

(41 citation statements)

References 20 publications

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“…Collagen is known to contribute to wound strength and therefore increased stiffness and strain in CTGF treated wounds is probably attributable to increased collagen IV in these tissues. These observations are consistent with similar findings for other growth factors such as PDGF, where treatment induced collagen IV and increased strength in corneal tissue [32] and in preclinical models of diabetic ulceration [33]. …”

Section: Discussionsupporting

confidence: 91%

Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role for CTGF in Human Diabetic Foot Ulcer Healing

Henshaw

Boughton

et al. 2015

Journal of Diabetes Research

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Aims/Hypothesis. Topical application of CTGF/CCN2 to rodent diabetic and control wounds was examined. In parallel research, correlation of CTGF wound fluid levels with healing rate in human diabetic foot ulcers was undertaken. Methods. Full thickness cutaneous wounds in diabetic and nondiabetic control rats were treated topically with 1 μg rhCTGF or vehicle alone, on 2 consecutive days. Wound healing rate was observed on day 14 and wound sites were examined for breaking strength and granulation tissue. In the human study across 32 subjects, serial CTGF regulation was analyzed longitudinally in postdebridement diabetic wound fluid. Results. CTGF treated diabetic wounds had an accelerated closure rate compared with vehicle treated diabetic wounds. Healed skin withstood more strain before breaking in CTGF treated rat wounds. Granulation tissue from CTGF treatment in diabetic wounds showed collagen IV accumulation compared with nondiabetic animals. Wound α-smooth muscle actin was increased in CTGF treated diabetic wounds compared with untreated diabetic wounds, as was macrophage infiltration. Endogenous wound fluid CTGF protein rate of increase in human diabetic foot ulcers correlated positively with foot ulcer healing rate (r = 0.406; P < 0.001). Conclusions/Interpretation. These data collectively increasingly substantiate a functional role for CTGF in human diabetic foot ulcers.

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Section: Discussionsupporting

confidence: 91%

Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role for CTGF in Human Diabetic Foot Ulcer Healing

Henshaw

Boughton

et al. 2015

Journal of Diabetes Research

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“…But because of the complex interaction among many angiogenic factors, it will not achieve satisfied therapeutic angiogenesis by targeting a single growth factor. In diabetic wound healing model, previous studies showed that Ang-1 (Bitto et al, 2008;Cho et al, 2006), PDGF-BB (Cheng et al, 2007;Li et al, 2008) and insulin-like growth factor-1(IGF-1) (Rabinovsky and DraghiaAkli, 2004) may ameliorate delayed wound repair through enhancing angiogenesis. The effect of these factors on decreased diabetic cardiovascular angiogenesis has not been reported.…”

Section: Discussionmentioning

confidence: 99%

Differential analyses of angiogenesis and expression of growth factors in micro- and macrovascular endothelial cells of type 2 diabetic rats

et al. 2009

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“…Moreover, cell cultures were recorded during 24 h by microscope (Biostation IM-Q, Nikon) in order to observe cell migration and proliferation. blood glucose levels exceeded 300 mg/dl (Li, et al 2008). …”

Section: In Vitro Migration Assaymentioning

confidence: 98%

“…The wounds were excised in 5 µm thick layers under the fascia, including a 5 mm margin of unwounded skin, bisected and fixed in 10% formalin for 24 hours. The samples were processed by H&E staining for morphological observations (Li, et al 2008;Ulubayram, et al 2001). …”

Section: Histological Analysismentioning

confidence: 99%

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

Gainza

Aguirre

Pedraz

et al. 2013

European Journal of Pharmaceutical Sciences

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Diabetic foot ulcers (DFUs) represent a major clinical challenge in the ageing population. To address this problem, rhEGF-loaded Poly-Lactic-co-Glycolic-Acid (PLGA)-Alginate microspheres (MS) were prepared by a modified w/o/w-doubleemulsion/solvent evaporation method. Different formulations were evaluated with the aim of optimising MSs properties by adding NaCl to the surfactant solution and/or the solvent removal phase and adding alginate as a second polymer. The characterization of the developed MS showed that alginate incorporation increased the encapsulation efficiency (EE) and NaCl besides increasing the EE also became the particle surface smooth and regular. Once the MS were optimised, the target loading of rhEGF was increased to 1% (PLGA-Alginate MS), and particles were sterilised by gamma radiation to provide the correct dosage for in vivo studies. In vitro cell culture assays demonstrated that neither the microencapsulation nor the sterilisation process affected rhEGF bioactivity or rhEGF wound contraction. Finally, the MS were evaluated in vivo for treatment of the full-thickness wound model in diabetised Wistar rats. rhEGF MS treated animals showed a statistically significant decrease of the wound area by days 7 and 11, a complete re-epithelisation by day 11 and an earlier resolution of the inflammatory process. Overall, these findings demonstrate the promising potential of rhEGF-loaded MS (PLGA-Alginate MS) to promote faster and more effective wound healing, and suggest its possible application in DFU treatment.

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Research of PDGF-BB Gel on the Wound Healing of Diabetic Rats and Its Pharmacodynamics

Cited by 71 publications

References 20 publications

Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role for CTGF in Human Diabetic Foot Ulcer Healing

Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role for CTGF in Human Diabetic Foot Ulcer Healing

Differential analyses of angiogenesis and expression of growth factors in micro- and macrovascular endothelial cells of type 2 diabetic rats

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

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