The research is based on the tasks of applied research on the topic: «Development of a complex of energy-efficient and resource-saving equipment and promising technologies for feeding farm animals of the AIC of Ukraine», state registration number 0121U108589. The authors’ research is aimed at solving current problems of technological renewal and development of the agro-industrial complex of Ukraine.The mechanism of action of probiotics is that they interfere with the development of pathogenic microflora, and can also synthesize biologically active substances (BAS – vitamins, amino acids, enzymes), while increasing digestibility and nutrient utilization. Probiotic microorganisms create a physical barrier between the cells of the intestinal epithelium and its contents. In addition, probiotic bacteria produce short-chain fatty acids, which leads to lower pH levels.In the conditions of the scientific farm of the Faculty of Technology of Production and Processing of Livestock and Veterinary Products of Vinnytsia National Agar University, research was conducted on the use of probiotic feed additives of natural origin in feeding broiler chickens. Rational use of biologically active substances in animal feed makes it possible to significantly increase the rate of absorption of feed nutrients, productivity and conservation of poultry. The article presents the results of research on the use of feed additives of natural origin in the feeding of broiler chickens. Broiler chickens, compound feed, meat, and blood were used as the object of the study. It has been established that the probiotic in the compound feed has a positive effect on the intensity of growth by increasing the digestibility of the main nutrients of the feed. It was found that the additive under study improves the quality of poultry meat due to the higher content of essential amino acids. New data have been obtained on the effect of feed additives on the availability of amino acids and the retention of mineral elements. For the first time, the dependence of physicochemical parameters, amino acid, fatty acid and mineral composition of the pectoral and femoral muscles on the ratio of different doses of the probiotic was found. An increase in the slaughter rates of broiler chickens under the action of a probiotic supplement has been established. The influence of the studied additive on the morphological, biochemical parameters of poultry blood was studied. Studies have shown a positive effect on the productivity, growth and quality of broiler meat.