As a new learning application form of self-regulated learning, informal learning and mobile learning, microlecture appears special content carrier in online educational ecological system, which becomes as focus topic on account of its characteristics-adapting to many applications and suited for different needs of learners. This paper proposed a method of knowledge point object microlecture design based on learning knowledge point under self-regulated learning environment. Component logic relation of microlecture resource was analyzed, and inspection rules of knowledge point objectified microlecture resource structure was developed. In order to improve the learners' learning efficiency, promote the construction of self-regulated learning, informal learning and mobile learning ,the rules can provide effective learning sequences and paths for learner by self-regulated learning algorithm, which is elaborated by the case of self-regulated microlecture environment on mathematics in middle school.