To address the issue that the influence of body-thread on the acceleration signal characteristics in penetration fuze is ambiguous, an accurate modeling method of body-thread is proposed in the finite element method (FEM). The body-thread element segments are obtained by the three-dimensional entity thread model, the initial layer nodes set and elements on the outer surface of the thread are established, and the initial layer elements are copied, rotated, and translated. Repeat this transformation, and get a complete thread model. This method is applied to simulation analysis of a warhead penetrating 4-layer concrete plates, and the influence of thread connection on acceleration signal characteristics in penetration fuze is studied. By comparing the acceleration signal and frequency spectrum of the proposed thread model with that of the simplified thread model, the amplitude of the acceleration signal of the proposed one is smaller than that of the simplified one in some certain frequency spectrum. It shows that the thread connection structure has with damping effect and effectively reduces the high-frequency interference of acceleration signal in penetration fuze.