Research on Enzyme Inhibition Potential and Phenolic Compounds From Origanum Vulgare Ssp. Vulgare

Abstract

This study assessed for the first time, the enzyme inhibitory properties of the Origanum vulgare ssp. vulgare extract. The main compounds were analysed chromatographically and spectrophotometrically. The enzymatic inhibitory potential was evaluated in vitro, against enzymes that play key roles in different diseases: acetylcholinesterase, urease, tyrosinase, trypsin and xanthine oxidase. A specific phenolic profile was evidenced, with large amounts of rosmarinic acid and luteolin 7-Oglucuronide; two phenolic diterpenes (rosmanol and rosmadial) were identified for the first time in this species. The oregano extract exhibited significant urease, tyrosinase and xanthine oxidase inhibitory effects, in line with the phenolic content. As a result of this study, new potential biological activities have been described for Origanum vulgare extract, which can be further studied and used in the pharmaceutical field. Rezumat În acest studiu se evaluează pentru prima dată potenţialul inhibitor enzimatic al extractului de Origanum vulgare ssp. vulgare (șovârf). Principalii compuşi chimici au fost analizaţi prin metode cromatografice şi spectrofotometrice. Capacitatea inhibitorie a fost evaluată in vitro, asupra unor enzime implicate în anumite afecţiuni: acetilcolinesteraza, ureaza, tirozinaza, tripsina şi xantin-oxidaza. În ceea ce priveşte profilul fenolic al extractului, s-au pus în evidenţă cantităţi mari de acid rozmarinic şi luteolin-7-O-glucuronidă; două diterpene fenolice (rosmanol şi rosmadial) au fost identificate pentru prima dată în această specie. Extractul de șovârf a inhibat semnificativ ureaza, tirozinaza şi xantin-oxidaza, în concordanţă cu conţinutul mare de compuşi polifenolici. Prin acest studiu s-au semnalat noi activităţi biologice potenţiale ale extractului de Origanum vulgare, care deschid noi perspective de interes ştiinţific în domeniul farmaceutic.

Evaluation of Hepatoprotective Activity and Oxidative Stress Reduction of Rosmarinus officinalis L. Shoots Tincture in Rats with Experimentally Induced Hepatotoxicity

“…Recording of chromatograms was performed at λ = 280 and 340 nm. The data acquisition was carried out with the Agilent ChemStation software [48,49].…”

Chemical Profile, Cytotoxic Activity and Oxidative Stress Reduction of Different Syringa vulgaris L. Extracts

“…Chromatograms were recorded at λ = 280 and 340 nm. Data acquisition was performed using the Agilent ChemStation software [ 42 , 43 ].…”

Comparative Chemical and Biological Activity of Selective Herbal Extracts

“…Chemical evaluation of the plant extracts Determination of total flavonoids in the plant extracts. The total flavonoid contents were determined spectrophotometrically [9,12,13,20]. Briefly, 0.5 mL of 2% solution of AlCl 3 in methanol was mixed with the same volume of extract (1 mg/mL).…”

“…Evaluation of antioxidant and enzyme inhibitory activity DPPH scavenger assay. The capability of selective extracts to scavenge DPPH radicals was assessed by a commonly used spectrophotometric method [9,13,20,24,25]. The solutions of each investigated sample were prepared in methanol, diluted with DMSO and added to 1 mL of DPPH methanolic solution (with an initial absorbance of 1.15).…”

“…The representatives of Lamiaceae family (Salvia, Lavandula, Mentha, Origanum etc.) are aromatic species, known for their flavoring properties due to essential oil [5,13,21,25]. Teucrium genus is not as known and investigated as other Lamiaceae representatives, but some of its variations are presently used as medicinal (Teucrium chamaedrys L., T. montanum L.) or food spices (Teucrium polium L.) in many traditional communities throughout the world (Greece, Spain, Turkey, Iran, Morocco, etc.).…”