Research on evolution process of full‐layer incision of skin tissue under different laser incidences

Abstract

Considering difficulties of achieving vertical incidence of beam in different positions of skin, it is significant to study potential effects of incidence angles of laser on incisions. Surgical platform with a 1064 nm continuous fiber laser was established. Incident angle was adopted and real‐time temperature fluctuations in laser operating area could be monitored. The rats were treated with laser at day 0 and day 3 after incision modeling, and H&E, Masson, Sirius Red, and Immuno‐histochemical staining and enzyme‐linked immunosorbent assay were adopted at day 3, 7, 14 to analyze the performance of healing. Laser with energy density of 67.54 J/mm2 can effectively accelerate wound healing in vivo, in which a laser with incident angle around 60° can effectively avoid scar hyperplasia. Therefore, the use of low energy laser with a small deflection angle has a good clinical application prospect in promoting wound healing.

Accelerating the remodeling of collagen in cutaneous full‐thickness wound using FIR soldering technology with bio‐targeting nanocomposites hydrogel

“…By referring to previous experiments, we have determined optimized fundamental parameters for the laser such as power level, scanning path, defocus amount, wavelength selection, and scanning speed [20,21]. Through thorough analysis of both macroscopic and microscopic aspects of skin tissue after performing laser welding procedures while considering factors like body temperature and thickness in rats' subjects; we further improved these parameters to achieve optimal results as presented in Table 1 [30].…”