Research on miRNA-195 and target gene CDK6 in oral verrucous carcinoma

Deng, Zhili; Wang, Y; Fang, Xiaodan; Yan, Fei; Pan, Huixing; Gu, Liqun; Xie, Changqing; Li, Y; Hu, Yingbin; Cao, Yukun; Tang, Zhangui

doi:10.1038/cgt.2017.18

Cited by 21 publications

(15 citation statements)

References 27 publications

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“…A study suggested a negative relationship between miR-195 and CDK6 and further verified that miR-195 and its target gene CDK6 could be regarded as potential diagnostic biomarkers and therapeutic targets of OVC. 146 These results suggested that miR-195 could serve as a potential therapeutic target and promising biomarker in head and neck cancer. Thus, miR-195 merits further exploration in future studies.…”

Section: Mir-195 In Various Cancersmentioning

confidence: 91%

MicroRNA-195: a review of its role in cancers

Liang

et al. 2018

OTT

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MicroRNAs (miRNAs) are small and highly conserved noncoding RNAs that regulate gene expression at the posttranscriptional level by binding to the 3′-UTR of target mRNAs. Recently, increasing evidence has highlighted their profound roles in various pathological processes, including human cancers. Deregulated miRNAs function as either oncogenes or tumor suppressor genes in multiple cancer types. Among them, miR-195 has been reported to significantly impact oncogenicity in various neoplasms by binding to critical genes and signaling pathways, enhancing or inhibiting the progression of cancers. In this review, we focus on the expression of miR-195 in regulatory mechanisms and tumor biological processes and discuss the future potential therapeutic implications of diverse types of human malignancies.

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Section: Mir-195 In Various Cancersmentioning

confidence: 91%

MicroRNA-195: a review of its role in cancers

Liang

et al. 2018

OTT

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“…NUF2 is a direct target of miR-3613-3p which participate in cell proliferation and cell cycle in hepatocellular carcinoma. CDK6 has been found as a target of several miRNAs, including miRNA-195, miRNA-29c, miRNA-218, miRNA-504, and miR-6883, and linked to bladder cancer, colon cancer, oral verrucous carcinoma, and medulloblastoma 61–67 . USP47 was found as a target of miRNA-204-5p and involves in gastric cancer cell proliferation 68 .…”

Section: Discussionmentioning

confidence: 99%

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology

Shen

Li²,

Teng

et al. 2019

Sci Rep

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MicroRNAs (miRNAs) bind to the 3ʹ-untranslated region of target mRNAs in a sequence-specific manner and subsequently repress gene translation. Human miR-26a has been studied extensively, but the target transcripts are far from complete. We first employed the CRISPR-Cas9 system to generate an miR-26a-knockout line in human cervical cancer HeLa cells. The miR26a-knockout line showed increased cell growth and altered proliferation. Proteomics technology of sequential window acquisition of all theoretical mass spectra (SWATH-MS) was utilized to compare the protein abundance between the wild-type and the knockout lines, with an attempt to identify transcripts whose translation was influenced by miR-26a. Functional classification of the proteins with significant changes revealed their function in stress response, proliferation, localization, development, signaling, etc. Several proteins in the cell cycle/proliferation signaling pathway were chosen to be validated by western blot and parallel reaction monitoring (PRM). The satisfactory consistency among the three approaches indicated the reliability of the SWATH-MS quantification. Among the computationally predicted targets, a subset of the targets was directly regulated by miR-26a, as demonstrated by luciferase assays and Western blotting. This study creates an inventory of miR-26a-targeted transcripts in HeLa cells and provides fundamental knowledge to further explore the functions of miR-26a in human cancer.

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“…Furthermore, the downregulation of miRNAs contributes to the pathogenesis of OVC. Specifically, miR-195 is found to be significantly downregulated in OVC [109] . miR-195 serves as either an oncogene or a tumor Figure 8.…”

Section: Malignant Cell Growthmentioning

confidence: 96%

“…miR-195 exerts its effects through various target genes, one of which is cdk6. cdk6 is activated in the G1 phase of the cellcycle and plays a role in the development of various cancers; cdk6 is found to be significantly upregulated in OVC [110] . This negative correlation between the expression of cdk6 and miR-195 in OVC serves as confirmation of the interaction between the two.…”

Section: Malignant Cell Growthmentioning

confidence: 99%

Overview of genetic signaling pathway interactions within cutaneous malignancies

Maner¹,

Dupuis²,

Su³

et al. 2020

JCMT

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Melanoma and non-melanoma cutaneous malignancies are some of the leading causes of cancer-related death in the United States. Though melanoma is more known to have a high mortality rate, the total mortality per year is nearly equal for between melanoma and non-melanoma skin cancer. Moreover, the non-melanoma types of cutaneous malignancies have potential to become locally invasive and even metastasize with very little to no treatment options when advanced. The development of these malignancies involves various genetic pathways through the four hallmarks of cancer development: malignant cell growth, apoptosis evasion, the use of supporting stroma and vascularization, and modulating and promoting an inadequate immune response. The genetic signaling pathways of basal cell carcinoma, squamous cell carcinoma, verrucous carcinoma, basosquamous cell carcinoma, melanoma, and cutaneous T-cell lymphoma interact with each other through genetic predisposition as well as with environmental exposures. Furthermore, solar ultraviolet radiation and chronic inflammatory states are found to initiate the progression of many of these cutaneous malignancies. This paper includes validated models of genetic pathways, emerging pathways, and crosstalk between genetic pathways through the four hallmarks of cancer development. Moreover, unlike most reviews addressing oncogenetics of the well-recognized, as well as newly dynamic, interrelated, interactive, complex, and adaptive flux states.

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Research on miRNA-195 and target gene CDK6 in oral verrucous carcinoma

Cited by 21 publications

References 27 publications

MicroRNA-195: a review of its role in cancers

MicroRNA-195: a review of its role in cancers

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology

Overview of genetic signaling pathway interactions within cutaneous malignancies

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