In this research, the effect of two different per centages of silicon, i.e. 0.08 and 0.3 wt-%, on microstructure and stress rupture life of a Ni-based superalloy was investigated. The results showed that the fraction of eutectic increased from 1.86 to 3.55% with increasing Si content and a higher fraction of eutectic was found on grain boundaries in high Si-bearing alloy. Upon stress rupture at 975°C/225 MPa, cracking initiates and propagates along Laves phases in 0.3 wt-% Si alloy, causing shorter lifetime comparing to that of 0.08 wt-% Si. The microscopic examination uncovered that Si prefers to segregate towards liquid and γ phase, which can explain different rupture lifetimes of two groups of Si-bearing alloys.