This article designs and exploits the multilayer distributed mixed virtual network laboratory (Virtual NetLab) based on LabVIEW, and presents a new scheme of data acquisition which combines LabVIEW with OPC technology. This scheme primly solves the laboratory's difficult problem of data collection. Moreover, the remote-client of Virtual NetLab is enriched by the LabVIEW's strong network function. The experiment results show that the results of the remote client experiment and the running results of the local experimental system are consistent. And the delay time is less than 1s. The function of remote synchronous experiment is realized. At the same time, it proves that the control algorithm has good control effect, which proves the availability of the virtual network lab and the good operation performance.