The collective motion of biological species has robust and flexible characteristics. Since the individual of the biological group interacts with other neighbors asymmetrically, which means the pairwise interaction presents asymmetrical characteristics during the collective motion, building the model of the pairwise interaction of the individual is still full of challenges. Based on deep learning (DL) technology, experimental data of the collective motion on Hemigrammus rhodostomus fish are analyzed to build an individual interaction model with multi-parameter input. First, a Deep Neural Network (DNN) structure for pairwise interaction is designed. Then, the interaction model is obtained by means of DNN proper training. We propose a novel key neighbor selection strategy, which is called the Largest Visual Pressure Selection (LVPS) method, to deal with multi-neighbor interaction. Based on the information of the key neighbor identified by LVPS, the individual uses the properly trained DNN model for the pairwise interaction. Compared with other key neighbor selection strategies, the statistical properties of the collective motion simulated by our proposed DNN model are more consistent with those of fish experiments. The simulation shows that our proposed method can extend to large-scale group collective motion for aggregation control. Thereby, the individual can take advantage of quite limited local information to collaboratively achieve large-scale collective motion. Finally, we demonstrate swarm robotics collective motion in an experimental platform. The proposed control method is simple to use, applicable for different scales, and fast for calculation. Thus, it has broad application prospects in the fields of multi-robotics control, intelligent transportation systems, saturated cluster attacks, and multi-agent logistics, among other fields.