Research on the remineralization of partially decalcified enamel surfaces using extracted tooth with calcifying solution

Abstract

OBJECTIVE: The recovery of tooth enamel after acid etching was examined in vitro using enamel powder in order to maximize the contact surface area. METHODS: The weight of the powder decreased due to acid etching and then increased when the powder was exposed to a calcifying solution. The increase in weight was due to the formation of needle like crystals. According to thermal gravimetric analysis, the newly formed enamel layer was very stable, and was suggested to be apatite by X-ray diffraction and FT-Raman analysis. There was no distinct difference between the newly formed layer and the original tooth in terms of composite X-ray image (CaK␣ and PK␣). The new layer appeared to be a crystallized extension of the tooth enamel, as determined from SEM observations. However, the outer layer of tooth enamel was damaged by acid etching, and the crystallization had deteriorated 48 hours later when compared with that of a non-etched sample. On the other hand, we were unable to confirm the effectiveness of the recovery. From these results, the recovery of damage caused by acid etching cannot be expected when a tooth is exposed to a calcifying solution. However, the finding demonstrated that the crystallized layer was apatite. and these were analyzed by Fourier Transform Raman Spectrophotometry (FT-Raman) 8) and by X-ray diffraction and by Thermogravimetry. Materials and Methods Materials Preparation of the extracted teethFive maxillary central incisors, extracted by periodontosis and stored in distilled water, without macroscopic dental caries, morphological abnormality, or calcification abnormality on the labial surface were used. Polishing was carried out with emery abrasive paper #400, after the labial surface was cleaned by a polishing brush. Preparation of enamel powderHuman enamel powder (hereafter abbreviated as enamel powder) was prepared from 20 sound permanent teeth. The tooth surfaces were cleaned with a polishing brush. Then the dentin was ground off