Research potential of multi-lineage chicken amniotic mesenchymal stem cells

Abstract

Amniotic mesenchymal stem cells (AMSCs) express octamer binding transcription factor 4 (Oct-4), which is necessary for maintaining the undifferentiated state of pluripotent stem cells. AMSCs also express CD29, CD44 and vimentin, which are specific markers of mesenchymal cells. We studied the biological characteristics and potential for cell therapy of AMSCs derived from 8-day-old chicken embryos. We induced the AMSCs to differentiate into adipocytes, osteoblasts and myocardial cells and used immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR) assays to detect the expressions of specific markers of AMSCs and differentiated cells. To assess the differentiation capacity of AMSCs, passage four cells were induced to differentiate into adipocytes, osteoblasts and myocardial cells. These results suggested that AMSCs isolated from chicken embryos exhibited the characteristics of multipotent stem cells. AMSCs, therefore, may be potential candidates for cellular transplantation therapy and tissue engineering.

Biological characterization of sheep kidney-derived mesenchymal stem cells

“…7B). Adipogenic differentiation of MMSCs was detected by Oil Red O staining (3). The MMSCs cultured in complete medium as a control group were negative for Oil Red O staining (Fig.…”

“…Mesenchymal stem cells (MSCs) possess a strong proliferative capacity and are able to differentiate into endothelium (1), adipocytes (1–3), neurons (2,4), osteoblasts (1,3,5) and myoblasts (5,6), which were first identified in bone marrow. In recent years, MSCs have been discovered in amniotic fluid, muscles, umbilical cord blood, dermis and other tissues (2,7–9).…”

Isolation and characterization of buffalo (<i>bubalus bubalis</i>) amniotic mesenchymal stem cells derived from amnion from the first trimester pregnancy

“…Evidence indicates that using bone marrow MSCs as nuclear donors in somatic cell nuclear transfer (SCNT) increases the developmental competence of porcine and bovine cloned embryos [ 3 , 22 ]. So far, AMSCs from different species have been reported, including human [ 16 ], horse [ 21 ], bovine [ 4 ], sheep [ 25 ], canine [ 9 ], chicken [ 23 ], porcine [ 19 ] and cat [ 37 ]. In buffalo, owing to its characteristics of heat resistance, high humidity resistance and strong disease resistance, it has tremendous potential development capabilities in agriculture across the globe.…”

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“…33 AMSCs are multipotent cells and express transcription factors which are essential for maintaining the self-renewal and undifferentiated state (Table 1). AMSCs are able to give rise into osteoblasts, adipocytes, myocardial cells, 34 neural-like cells, and islet like cells. 33 AMSCs suitable tool for tissue engineering research and autologous cell therapy.…”

“…33 AMSCs suitable tool for tissue engineering research and autologous cell therapy. [34][35][36] Avian cartilage stem/progenitor cells Avian cartilage-derived stem/progenitor cells (CSPCs) were obtained from the surface zone of articular cartilage from 20days old chicken embryo. CSPCs are uniform population with long fusiform or polygon morphology.…”