Research progress in gene editing technology

Huang, Sheng; Yao, Yan; Su, Fei; Huang, Xiaorui; Xia, Dandan; Jiang, Xue; Dong, Yan; Lv, Ping; Chen, Fangyuan; Lv, Yanwei

doi:10.52586/4997

Cited by 13 publications

(4 citation statements)

References 134 publications

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“…Gene editing is an emerging molecular biology technique that involves artificially altering a specific gene locus to modify the expression characteristics of the target gene, facilitating the study of gene function ( Huang et al, 2021 ). CRISPR-Cas9 is a novel gene editing technology that utilizes an RNA-mediated adaptive immune defense system first discovered in 1987 ( Ishino et al, 1987 ).…”

Section: New Technologies For Organoid Constructionmentioning

confidence: 99%

The application of organoids in colorectal diseases

Liu,

Wang,

Luan

et al. 2024

Front. Pharmacol.

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Intestinal organoids are a three-dimensional cell culture model derived from colon or pluripotent stem cells. Intestinal organoids constructed in vitro strongly mimic the colon epithelium in cell composition, tissue architecture, and specific functions, replicating the colon epithelium in an in vitro culture environment. As an emerging biomedical technology, organoid technology has unique advantages over traditional two-dimensional culture in preserving parental gene expression and mutation, cell function, and biological characteristics. It has shown great potential in the research and treatment of colorectal diseases. Organoid technology has been widely applied in research on colorectal topics, including intestinal tumors, inflammatory bowel disease, infectious diarrhea, and intestinal injury regeneration. This review focuses on the application of organoid technology in colorectal diseases, including the basic principles and preparation methods of organoids, and explores the pathogenesis of and personalized treatment plans for various colorectal diseases to provide a valuable reference for organoid technology development and application.

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Section: New Technologies For Organoid Constructionmentioning

confidence: 99%

The application of organoids in colorectal diseases

Liu,

Wang,

Luan

et al. 2024

Front. Pharmacol.

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“…The DNA-binding domain in these systems is specially designed to attach to the specific DNA sequence that the DNA nuclease domain cleaves. However, due to their high cost, high off-target likelihood, and complex structure, these first- and second-generation gene editing technologies’ wide range of applications suffer from a low target recognition rate (Huang S. et al, 2021 ), and now they are replaced by CRISPR/Cas9 which relies on Cas9 and a single guide RNA (sgRNA) to achieve precise cutting to offer ease of design, development and increased efficacy (Kantor et al, 2020 ). Prior to the introduction of gene editing technologies, natural, physical, or chemical mutagenesis and random insertion of transgenic DNA were the principal approaches used to generate mutations within targeted cells.…”

Section: Progress In Gene Editing Techniquesmentioning

confidence: 99%

Gene editing in monogenic autism spectrum disorder: animal models and gene therapies

Wang

Lv²,

Huang³

et al. 2022

Front. Mol. Neurosci.

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Autism spectrum disorder (ASD) is a lifelong neurodevelopmental disease, and its diagnosis is dependent on behavioral manifestation, such as impaired reciprocal social interactions, stereotyped repetitive behaviors, as well as restricted interests. However, ASD etiology has eluded researchers to date. In the past decades, based on strong genetic evidence including mutations in a single gene, gene editing technology has become an essential tool for exploring the pathogenetic mechanisms of ASD via constructing genetically modified animal models which validates the casual relationship between genetic risk factors and the development of ASD, thus contributing to developing ideal candidates for gene therapies. The present review discusses the progress in gene editing techniques and genetic research, animal models established by gene editing, as well as gene therapies in ASD. Future research should focus on improving the validity of animal models, and reliable DNA diagnostics and accurate prediction of the functional effects of the mutation will likely be equally crucial for the safe application of gene therapies.

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“…1 Compared to traditional methods, gene editing technology is more efficient, feasible, and manageable. 2 Additionally, gene mutations introduced through gene editing are similar to natural mutations, making them more acceptable compared to genetically modified organisms (GMOs). In recent years, gene editing technology has been widely applied in various fields such as crop improvement 3 and disease treatment.…”

Section: Introductionmentioning

confidence: 99%