Research progress of aptasensor technology in the detection of foodborne pathogens

He, Yue; Yuan, Jin-Peng; Khan, Imran Mahmood; Zhang, Lili; Ma, Peisheng; Wang, Zhouping

doi:10.1016/j.foodcont.2023.109891

Cited by 21 publications

(3 citation statements)

References 92 publications

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“…Aptasensors provide rapid and sensitive detection of various pathogens [20,[35][36][37][38]. Aptamers are compatible with a huge variety of analytical techniques.…”

Section: Discussionmentioning

confidence: 99%

Nanoisland SERS-Substrates for Specific Detection and Quantification of Influenza A Virus

Zhdanov,

Gambaryan,

Akhmetova

et al. 2023

Biosensors

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Surface-enhanced Raman spectroscopy (SERS)-based aptasensors for virus determination have attracted a lot of interest recently. This approach provides both specificity due to an aptamer component and a low limit of detection due to signal enhancement by a SERS substrate. The most successful SERS-based aptasensors have a limit of detection (LoD) of 10–100 viral particles per mL (VP/mL) that is advantageous compared to polymerase chain reactions. These characteristics of the sensors require the use of complex substrates. Previously, we described silver nanoisland SERS-substrate with a reproducible and uniform surface, demonstrating high potency for industrial production and a suboptimal LoD of 4 × 105 VP/mL of influenza A virus. Here we describe a study of the sensor morphology, revealing an unexpected mechanism of signal enhancement through the distortion of the nanoisland layer. A novel modification of the aptasensor was proposed with chromium-enhanced adhesion of silver nanoparticles to the surface as well as elimination of the buffer-dependent distortion-triggering steps. As a result, the LoD of the Influenza A virus was decreased to 190 VP/mL, placing the nanoisland SERS-based aptasensors in the rank of the most powerful sensors for viral detection.

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“…Aptasensors provide rapid and sensitive detection of various pathogens [20,[35][36][37][38]. Aptamers are compatible with a huge variety of analytical techniques.…”

Section: Discussionmentioning

confidence: 99%

Nanoisland SERS-Substrates for Specific Detection and Quantification of Influenza A Virus

Zhdanov,

Gambaryan,

Akhmetova

et al. 2023

Biosensors

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“…pubs.aip.org/aip/adv with minimal lab infrastructure, though their sensitivity and accuracy can vary based on bioreceptor specificity and environmental conditions. 17 In recent years, there has been a widespread deployment of nucleic acid (aptamer) detection methods for accurate identification of foodborne pathogens. 17 Aptamers, single-stranded DNA or RNA molecules, exhibit remarkable stability under varying conditions, making them highly valuable in diagnostics, biosensing, and therapeutics.…”

Section: Articlementioning

confidence: 99%

“…17 In recent years, there has been a widespread deployment of nucleic acid (aptamer) detection methods for accurate identification of foodborne pathogens. 17 Aptamers, single-stranded DNA or RNA molecules, exhibit remarkable stability under varying conditions, making them highly valuable in diagnostics, biosensing, and therapeutics. Their small size and low immunogenicity provide significant advantages, while their rapid development and reproducibility make them appealing alternatives to antibodies.…”

Section: Articlementioning

confidence: 99%

3D printed microfluidic chip integrated with nanointerferometer for multiplex detection of foodborne pathogens

Feng,

Mo,

Song

2024

AIP Advances

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The current foodborne pathogen detection methods, such as culture-based methods, polymerase chain reaction, and optical and electrochemical biosensors with nucleic acid, have high sensitivity and selectivity. However, they are slow, expensive, and require well-trained operators. In this study, we utilized a 3D printer to develop a novel chip with an aptamer-based nanointerferometer capable of identifying four distinct foodborne pathogens: Listeria monocytogenes, Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus. The aptamer sensor on the chip achieved a limit-of-detection of 10 colony forming unit (CFU)/ml. With its high sensitivity and specificity, this chip offers a cost-effective platform for distinguishing and screening different foodborne pathogens.

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An aptamer magnetic capture based colorimetric method for rapid and sensitive detection of Listeria monocytogenes

Du,

Guo,

Liu

et al. 2024

Food Measure

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Research progress of aptasensor technology in the detection of foodborne pathogens

Cited by 21 publications

References 92 publications

Nanoisland SERS-Substrates for Specific Detection and Quantification of Influenza A Virus

Nanoisland SERS-Substrates for Specific Detection and Quantification of Influenza A Virus

3D printed microfluidic chip integrated with nanointerferometer for multiplex detection of foodborne pathogens

An aptamer magnetic capture based colorimetric method for rapid and sensitive detection of Listeria monocytogenes

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