Research progress on the structure and function of endomucin

Zhang, Guoxin; Yang, Xiubin; Gao, Ran

doi:10.1002/ame2.12142

Cited by 18 publications

(11 citation statements)

References 33 publications

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“…EMCN is a heavily O-glycosylated single-transmembrane sialomucin, expressed by venous and capillary rather than arterial endothelium. 30 The Western blotting assays showed that the iPSC-ECs under both normoxia and hypoxia expressed EMCN (Figure S2), which indicated that iPSC-ECs were more like venous and capillary endothelium. CXCR4 acts as the receptor for stromal cell-derived factor 1 (SDF1), which mediates the bone marrow homing of peripheral or transplanted CXCR4 + HSPCs.…”

Section: Resultsmentioning

confidence: 99%

Bioinspired Nanovesicles Convert the Skeletal Endothelium-Associated Secretory Phenotype to Treat Osteoporosis

Cui

Guo

et al. 2022

ACS Nano

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Recently, bone marrow endothelial cells (BMECs) were found to play an important role in regulating bone homeostasis. However, few studies utilized BMECs to treat bone metabolic diseases including osteoporosis. Here, we reported bioinspired nanovesicles (BNVs) prepared from human induced pluripotent stem cells-derived endothelial cells under hypoxia culture through an extrusion approach. Abundant membrane C-X-C motif chemokine receptor 4 conferred these BNVs bone-targeting ability and the endothelial homology facilitated the BMEC tropism. Due to their unique endogenous miRNA cargos, these BNVs re-educated BMECs to secret cytokines favoring osteogenesis and anti-inflammation. Owing to the conversion of secretory phenotype, the osteogenic differentiation of bone mesenchymal stem cells was facilitated, and the M1-macrophage-dominant pro-inflammatory microenvironment was ameliorated in osteoporotic bones. Taken together, this study proposed BMEC-targeting nanovesicles treating osteoporosis via converting the skeletal endothelium-associated secretory phenotype.

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Section: Resultsmentioning

confidence: 99%

Bioinspired Nanovesicles Convert the Skeletal Endothelium-Associated Secretory Phenotype to Treat Osteoporosis

Cui

Guo

et al. 2022

ACS Nano

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“…5k-l ). Endomucin (Emcn) is an integral membrane glycoprotein expressed in cECs and vECs 74 that modulates VEGF-induced angiogenesis in the developing retina 75 . DE analysis by scRNAseq revealed that Emcn expression was upregulated in EAE vECs ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Single-cell RNA-sequencing implicates venous endothelial cells as a source of VEGF-A-mediated neo-angiogenesis in neuroinflammation

Shahriar

Price

Wayne

et al. 2022

Preprint

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Histopathological studies of multiple sclerosis (MS), a demyelinating disease of the central nervous system (CNS), and its animal model experimental autoimmune encephalomyelitis (EAE) have found newly formed leaky vessels in demyelinated acute and chronic plaques, in addition to blood-brain barrier damage in existing vessels, that exacerbate disease pathology by increasing CNS infiltration of immune cells. However, which vessel subtypes and signaling pathways generate these aberrant vessels is poorly understood. Using single-cell RNA sequencing and in vivo validation, we find that transcriptome signatures of neo-angiogenesis arise in venous endothelial cells in both acute and chronic EAE, and correlate with upregulation in VEGF-A signaling. These neo-angiogenic markers are also increased in human MS lesions. Treatment with a VEGF-A blocking antibody ameliorates EAE pathology by reducing neo-angiogenic transcriptomic signatures in vivo, suggesting that VEGF-A drives angiogenesis from vein ECs in EAE. Our findings suggest novel therapies targeting the vasculature that could benefit chronic MS.

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“…It is further interesting to note that the expression of EMCN in the main capillary population increases slightly in time, unlike CD31 expression. EMCN is strongly involved in neovascularization [30], while CD31 plays an important role in intercellular junctions and the mechanisms of leukocyte transendothelial migration [31,32], especially for neutrophils and monocytes. When CD31 decreases, the migration of these cells through the capillaries to the tissues decreases [33].…”

Section: Discussionmentioning

confidence: 99%

Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium

et al. 2022

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Angiogenesis and bone regeneration are closely interconnected processes. Whereas type-H blood vessels are abundantly found in the osteogenic zones during endochondral long bone development, their presence in flat bones’ development involving intramembranous mechanisms remains unclear. Here, we hypothesized that type-H-like capillaries that highly express CD31 and Endomucin (EMCN), may be present at sites of intramembranous bone development and participate in the control of osteogenesis. A rabbit model of calvarial bone augmentation was used in which bone growth was controlled over time (2–4 weeks) using a particulate bone scaffold. The model allowed the visualization of the entire spectrum of stages throughout bone growth in the same sample, i.e., active ossification, osteogenic activity, and controlled inflammation. Using systematic mRNA hybridization, the formation of capillaries subpopulations (CD31–EMCN staining) over time was studied and correlated with the presence of osteogenic precursors (Osterix staining). Type-H-like capillaries strongly expressing CD31 and EMCN were identified and described. Their presence increased gradually from the regenerative zone up to the osteogenic zone, at 2 and 4 weeks. Type-H-like capillaries may thus represent the initial vascular support encountered in flat bones’ development and which organize osteogenic niches.

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Research progress on the structure and function of endomucin

Cited by 18 publications

References 33 publications

Bioinspired Nanovesicles Convert the Skeletal Endothelium-Associated Secretory Phenotype to Treat Osteoporosis

Bioinspired Nanovesicles Convert the Skeletal Endothelium-Associated Secretory Phenotype to Treat Osteoporosis

Single-cell RNA-sequencing implicates venous endothelial cells as a source of VEGF-A-mediated neo-angiogenesis in neuroinflammation

Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium

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