In order to analyze the characteristics of the photoacoustic signals of two identical cells at different distances, this paper conducted a finite element analysis on the influence of the parameters of the two-cell photoacoustic spectrum. The study established a two-cell model at different distances and a single cell model under the same peripheral environment. Using the two-cell model, we have obtained the frequency domain sound pressure level curves of the subject red blood cells at different angles under the influence of another cell at a different distance. In the single cell model, this paper obtains the frequency domain sound pressure level curves of a single cell at different angles when it is not affected. The frequency domain sound pressure level curve of each angle of the subject cell is compared with the frequency domain sound pressure level curve of a single cell at the same position. The results show that when the distance between the two cells is 26.6 µm, the frequency domain sound pressure level curve of the subject cell has the highest similarity with the frequency domain sound pressure level curve of a single cell. This study shows that when the distance between two cells is appropriate, the photoacoustic signals between the cells have the least influence on each other.