Despite the COVID-19 pandemic, the current era offers the ultimate possibility for prosperous corporate life, especially in the transport sector. Industry 4.0 covers artificial intelligence, big data, or industrial IoT, and thus spatial cognition algorithms, traffic flow prediction, autonomous vehicles, and smart sustainable mobility are not far away. The mentioned tools have already been implemented by enterprises in emerging countries. This exploration focused on transportation within the V4 region from 2016–2021. This article aims to confirm the positive sequel of applying Industry 4.0 to chosen indicators of profitability. The positive, negative, or no shift in the development of 534 businesses was based on Pettitt’s test. The Pearson chi-square test disclosed the significant dependency between Industry 4.0 and shifts in profitability ratios. Then, more than 25% of enterprises involved in Industry 4.0 had positive shifts in ROA, ROC, ROS, and ROR. The research proved not only its balanced effect but also its augmented force through the z-test of proportion. This investigation may provide multiple proofs for connected sectors with transportation to adapt the tools of Industry 4.0 and deliver the call for the governments in the V4 region to make this tool more achievable.