Quantitative and qualitative flesh production in the Silurus glanis species was comparatively studied between two fish groups: one from aquaculture (AG) and the other from a natural environment, the Prut River (RG). Morphometry was carried out on the fish, and then biometric and conformational indices were calculated. Better values were found in the aquaculture catfish. The Fulton coefficient was 0.82 in the Prut River fish and 0.91% in the farmed ones. The fleshy index reached 19.58% in the AG fish and 20.79% in the RG fish, suggesting better productive capabilities in the AG fish. Postslaughter, the flesh yield and its quality were assessed at different moments throughout the refrigeration period (0–15 days), and chemical compound loss occurred. In the AG samples, the water content decreased by 8.87%, proteins by 27.66%, and lipids by 29.58%. For the RG samples, the loss reached 8.59% in water, 25.16% in proteins, and 29%in lipids. By studying the fatty acids profile and sanogenic indices, good levels of PUFA (31–35%) were found, and the atherogenic index reached 0.35–0.41 while the thrombogenic index ranged between 0.22 and 0.27. Consequently, it can be stated that fish origin and especially the refrigeration period influence the flesh proximate composition and nutritional value of European catfish.