Research Techniques Made Simple: Stratum Corneum Tape Stripping

Keurentjes, Anne J.; Jakaša, Ivone; Kežić, Sanja

doi:10.1016/j.jid.2021.01.004

Cited by 32 publications

(21 citation statements)

References 21 publications

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“…TS uses adhesive tapes to collect successive layers of the SC following topical application of formulations and subsequently quantify the drug content in each layer of stripped skin. TS has been combined with analytical techniques to measure the penetration of chemicals to the SC over time and evaluate the efficacy of topical dermatological dosage forms [ 2 , 7 ]. This method can provide useful information about the distribution of topically applied substances in the skin; however, TS has been shown to be heavily influenced by a number of experimental variables, including the type of tape used, the pressure applied by the operator, the duration of the pressure, the contact time, and the velocity of tape removal, as well as the formulation components [ 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Dermal Delivery of Diclofenac Sodium—In Vitro and In Vivo Studies

Iliopoulos¹,

Goh

Haque³

et al. 2022

Pharmaceutics

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Previously, we reported the use of confocal Raman spectroscopy (CRS) as a novel non-invasive approach to determine drug disposition in the skin in vivo. Results obtained by CRS were found to correlate with data from the well-established in vitro permeation test (IVPT) model using human epidermis. However, these studies used simple vehicles comprising single solvents and binary or ternary solvent mixtures; to date, the utility of CRS for monitoring dermal absorption following application of complex marketed formulations has not been examined. In the present work, skin delivery of diclofenac sodium (DFNa) from two topical dermatological drug products, namely Diclac® Lipogel 10 mg/g and Primofenac® Emulsion gel 1%, was determined by IVPT and in vivo by both CRS and tape stripping (TS) methodologies under similar experimental conditions. The in vivo data were evaluated against the in vitro findings, and a direct comparison between CRS and TS was performed. Results from all methodologies showed that Diclac promoted significantly greater DFNa delivery to the skin (p < 0.05). The cumulative amounts of DFNa which permeated at 24 h in vitro for Diclac (86.5 ± 9.4 µg/cm2) were 3.6-fold greater than the corresponding amounts found for Primofenac (24.4 ± 2.7 µg/cm2). Additionally, total skin uptake of DFNa in vivo, estimated by the area under the depth profiles curves (AUC), or the signal intensity of the drug detected in the upper stratum corneum (SC) (4 µm) ranged from 3.5 to 3.6-fold greater for Diclac than for Primofenac. The shape of the distribution profiles and the depth of DFNa penetration to the SC estimated by CRS and TS were similar for the two methods. However, TS data indicated a 4.7-fold greater efficacy of Diclac relative to Primofenac, with corresponding total amounts of drug penetrated, 94.1 ± 22.6 µg and 20.2 ± 7.0 µg. The findings demonstrate that CRS is a methodology that is capable of distinguishing skin delivery of DFNa from different formulations. The results support the use of this approach for non-invasive evaluation of topical products in vivo. Future studies will examine additional formulations with more complex compositions and will use a wider range of drugs with different physicochemical properties. The non-invasive nature of CRS coupled with the ability to monitor drug permeation in real time offer significant advantages for testing and development of topical dermatological products.

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Section: Introductionmentioning

confidence: 99%

Dermal Delivery of Diclofenac Sodium—In Vitro and In Vivo Studies

Iliopoulos¹,

Goh

Haque³

et al. 2022

Pharmaceutics

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“…Subsequently, the findings have been confirmed by several studies [ 8 , 26 – 28 ]. Investigations of tape strips have also been widely used in dermatology to determine protein, lipid, and microbial expression noninvasively [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of cutaneous malignant melanoma using RNA sampled by tape strips: A study protocol

et al. 2022

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Background Cutaneous malignant melanoma (CMM) is curable if detected in its early stages. However, the clinical recognition of CMM is challenging. An American research group has shown promising results in detecting CMM based on RNA profiles sampled from suspicious lesions with tape strips. We aim to further develop this technique and validate if RNA profiles sampled with tape strips can detect CMM. Methods This prospective cohort study will include approximately 200 lesions clinically suspected of CMM requiring surgical removal. Tape stripping of the lesions will be performed just before surgical excision. Subsequently, RNA on the tape strips is analyzed using quantitative real-time polymerase chain reaction with TaqMan technology. The results are combined into a binary outcome where positive indicates CMM and negative indicates no CMM. The histopathological diagnosis of the lesions will be used as the gold standard. The main outcome is the results of the RNA test and the histopathological diagnosis, which, combined, provide the sensitivity and specificity of the test. Discussion The accuracy of the clinical examination in CMM diagnostics is limited. This clinical trial will explore the ability to use RNA analysis to improve the management of suspicious lesions by enhancing early diagnostic accuracy. Hopefully, it can reduce the number of benign lesions being surgically removed to rule out CMM and decrease patient morbidity. Trial registration The project was approved by The Committee on Health Research Ethics of the Capital Region of Denmark (H-15010559) and registered at the Danish Data Protection Agency (BFH-2015-065).

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“…At T3, SC tape stripping (D-Squame sampling) was done to collect SC samples. 21 Sampling was conducted using round adhesive disks (22-mm Standard D-Squame Skin Sampling Discs; Monaderm) which were pressed onto the test areas for 10 s with the D500 D-Squame Pressure Instrument (CuDerm) in order to ensure a defined pressure. This procedure was conducted with eight discs.…”

Section: Washing Proceduresmentioning

confidence: 99%

Effects of skin washing frequency on the epidermal barrier function and inflammatory processes of the epidermis: An experimental study

et al. 2022

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Background Increased hand hygiene measures during the COVID‐19 pandemic have led to an increased quantum of hand eczema (HE). Objectives To examine the effects of varying washing frequencies using current mild cleansing agents—alongside with the effect of a rehydrating cream—on the epidermal barrier function and inflammatory processes of the stratum corneum(SC). Methods Standardized skin washings on the volar aspects of the lower arms of skin‐healthy volunteers were performed using the automated cleansing device either 5 or 11 times within 4 h for 60 s each with a standard cleanser, a lipid‐containing syndet, or a lipid‐containing syndet followed by one‐time application of a rehydrating cream. Skin bioengineering parameters (transepidermal water loss, SC hydration, erythema, and SC pH) and biochemical/immunological parameters (interleukin‐1α, interleukin‐1α receptor antagonist and natural moisturizing factor) of SCsamples collected by tape stripping were assessed. Results All applied washing procedures provided comparable, mild effects on the epidermal barrier function and skin inflammation. Conclusion Occupational skin cleansers seem to have improved regarding skin barrier damaging effects. To further corroborate this, a study design, modified on the basis of our findings, applying longer washing periods for consecutive days seems desirable.

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Research Techniques Made Simple: Stratum Corneum Tape Stripping

Cited by 32 publications

References 21 publications

Dermal Delivery of Diclofenac Sodium—In Vitro and In Vivo Studies

Dermal Delivery of Diclofenac Sodium—In Vitro and In Vivo Studies

Detection of cutaneous malignant melanoma using RNA sampled by tape strips: A study protocol

Effects of skin washing frequency on the epidermal barrier function and inflammatory processes of the epidermis: An experimental study

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