A method for measuring vibration characteristics of MEMS (Micro Electro Mechanical System) is presented. This method aims to simulate a real environment where MEMS operates. At first, the method of applying high and low temperature in vacuum environment is studied. And the excitation method applying to movable microstructures of MEMS in this environment is found. Based on the above environmental conditions, the vibration characteristics of MEMS movable microstructure are measured by micro-laser vibration measurement. The base excitation method is used to measure the vibration characteristics of MEMS movable microstructures outside the plane. ANSYS 14.0 was used for finite element simulation to verify this method. Electrostatic excitation method is used to measure the inside of the plane. Stroboscopic method is used to verify the electrostatic excitation by fitting the displacement signal of the movable microstructure and the excitation output signal. The results show that the out-of-plane first-order frequency is 11.926 kHz, and the error is 0.30% compared with the experimental results. The amplitude is 44.218 nm, and the error is 0.59%. The in-plane first-order frequency is 5715.7Hz, which achieves the requirement of design precision. Both the numerical simulation and the stroboscopic method verify the excitation method well. The effect of temperature on the natural frequency of the structure is negatively correlated. And as the temperature drops, the motion of the structure becomes increasingly violent. The findings of this study provide important guidance for maintenance, reliable operation and optimal design of MEMS.