Reserpine

Slotkin, Theodore A.

doi:10.1007/978-1-4684-2943-5_1

Cited by 3 publications

(1 citation statement)

References 143 publications

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“…Reserpine interferes with the storage of aminergic neurotransmitters such as serotonin and dopamine (see reviews by Shore 1962;Slotkin 1974;Giachetti and Shore 1978;Johnson 1988). Injection of leeches with 100 ~tg reserpine causes a dramatic reduction in serotonin levels within the nervous system and nearly eliminates swimming activity in isolated nerve cords, an effect which can be reversed by bath application of 50 IxM serotonin (O'Gara et al 1991).…”

Section: Membrane Properties Of Cell 204 In Reserpine-treated Leechesmentioning

confidence: 99%

Modulation of swimming behavior in the medicinal leech

Angstadt

Friesen

1993

J Comp Physiol A

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The effects of serotonin on the electrical properties of swim-gating neurons (cell 204) were examined in leech (Hirudo medicinalis) nerve cords. Exposure to serotonin decreased the threshold current required to elicit swim episodes by prolonged depolarization of an individual cell 204 in isolated nerve cords. This effect was correlated with a more rapid depolarization and an increased impulse frequency of cell 204 in the first second of stimulation. In normal leech saline, brief depolarizing current pulses (1 s) injected into cell 204 failed to elicit swim episodes. Following exposure to serotonin, however, identical pulses consistently evoked swim episodes. Thus, serotonin appears to transform cell 204 from a gating to a trigger cell. Serotonin had little effect on the steady-state current-voltage relation of cell 204. However, serotonin altered the membrane potential trajectories in response to injected current pulses and increased the amplitude of rebound responses occurring at the offset of current pulses. These changes suggest that serotonin modulates one or more voltage dependent conductances in cell 204, resulting in a more rapid depolarization and greater firing rate in response to injected currents. Thus, modulation of intrinsic ionic conductances in cell 204 may account in part for the increased probability of swimming behavior induced by serotonin in intact leeches.

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Section: Membrane Properties Of Cell 204 In Reserpine-treated Leechesmentioning

confidence: 99%

Modulation of swimming behavior in the medicinal leech

Angstadt

Friesen

1993

J Comp Physiol A

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Chlorpyrifos releases norepinephrine from adult and neonatal rat brain synaptosomes

Dam

Seidler

Slotkin

1999

Developmental Brain Research

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SYNAPTIC VESICLES ISOLATED FROM RAT HEART:l‐[³H]NOREPINEPHRINE UPTAKE PROPERTIES¹

Bareis

Slotkin

1979

Journal of Neurochemistry

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Abstract— A fraction containing synaptic vesicles was isolated from rat heart by differential centrifugation, and the uptake of l‐[3H]norepinephrine was studied in vitro., Uptake was highly dependent upon time and temperature, and was linear for 6 min at 30° or 4 min at 37°C. About 80% of the measured uptake required both ATP and Mg2+ and was inhibited by nanomolar concentrations of reserpine; no inhibition was obtained with cocaine. These properties are characteristic of storage vesicle uptake as opposed to synaptic membrane uptake. Uptake of norepinephrine was saturable and displayed a single Km value of 2 μM. The uptake was completely stereospecific, as unlabeled dl‐norepinephrine was less than half as effective as unlabeled l‐norepinephrine in reducing uptake of l‐[3H]norepinephrine. Norepinephrine uptake could be inhibited by various phenethylamines and indoleamines following the rank order: reserpine > harmaline > 5‐hydroxytryptamine > dopamine > norepinephrine. The vesicle preparation also incorporated [3H]5‐hydroxytryptamine and [3H]dopamine. 5‐Hydroxytryptamine uptake displayed a Km of 0.5 μM and a maximal uptake equivalent to that seen with norepineph‐rine; dopamine uptake followed complex kinetics. Administration of reserpine in vivo or destruction of sympathetic neurons by long‐term guanethidine treatment both eliminated the ability of the preparation to take up norepinephrine. Synaptic vesicles of cardiac sympathetic neurons thus resemble vesicles prepared from other central and peripheral catecholaminergic tissues; this method may be used readily to examine drug effects on rat heart synaptic vesicle function.

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Reserpine

Cited by 3 publications

References 143 publications

Modulation of swimming behavior in the medicinal leech

Modulation of swimming behavior in the medicinal leech

Chlorpyrifos releases norepinephrine from adult and neonatal rat brain synaptosomes

SYNAPTIC VESICLES ISOLATED FROM RAT HEART:l‐[³H]NOREPINEPHRINE UPTAKE PROPERTIES¹

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Reserpine

Cited by 3 publications

References 143 publications

Modulation of swimming behavior in the medicinal leech

Modulation of swimming behavior in the medicinal leech

Chlorpyrifos releases norepinephrine from adult and neonatal rat brain synaptosomes

SYNAPTIC VESICLES ISOLATED FROM RAT HEART:l‐[3H]NOREPINEPHRINE UPTAKE PROPERTIES1

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SYNAPTIC VESICLES ISOLATED FROM RAT HEART:l‐[³H]NOREPINEPHRINE UPTAKE PROPERTIES¹