Reserve carbohydrates and lipids from the seeds of four tropical tree species with different sensitivity to desiccation

Mello, Juliana Iura de Oliveira; Barbedo, Claudio José; Salatino, Antônio; Figueiredo-Ribeiro, Rita de Cássia L.

doi:10.1590/s1516-89132010000400019

Cited by 39 publications

(30 citation statements)

References 42 publications

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“…As shown by Mello et al (2010), lipids in cotyledons of C. echinata represent more than 17 percent of DM, whereas linoleic acid represents nearly half of them (more than 45 percent). Respiration using carbohydrates produces respiratory quotient (RQ) close to 1.0 since carbohydrate oxidation (highly oxygenated substrates) involves equal amounts of carbon dioxide and oxygen.…”

Section: Discussionmentioning

confidence: 97%

“…These oxidative reactions could explain the low viability of C. echinata seeds while stored at temperatures higher than the freezing ones, even when they have low water content, as described by Barbedo et al (2002) and Hellmann et al (2006). Moreover, great amounts of linoleic acid were found in C. echinata cotyledons, (Mello et al 2010), which is a polyunsaturated fatty acid (PUFA) and is particularly susceptible to oxidation by singlet oxygen ( Our results provide evidence on the damage caused by oxidative reactions as revealed by tetrazolium tests performed on seeds based upon different hydration treatments as well as different temperatures. In this work, the target levels of controlled seed hydration covered two energetic states of water (Vertucci & Farrant 1995).…”

Section: Discussionmentioning

confidence: 99%

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Short storability of Caesalpinia echinata Lam. seeds as a consequence of oxidative processes

Lamarca

Barbedo

2012

Hoehnea

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-(Short storability of Caesalpinia echinata Lam. seeds as a consequence of oxidative processes). The seed bank is one of the strategies for the preservation of endangered species, such as Caesalpinia echinata Lam. In this work we studied the changes in O 2 consumption and CO 2 release by seeds incubated at different temperatures and water contents, evaluating the deterioration of seeds through germination and tetrazolium tests. Our results demonstrated that the deterioration processes occurring in C. echinata seeds are related to respiration and possibly other oxidative processes, causing the death of embryonic tissues in short periods and loss of seed viability. This characteristic means that seed bank is an alternative for Caesalpinia echinata conservation, however it depends on the control of these oxidative processes. Key words: brazilwood, deterioration, seed respiration, storage RESUMO -(Baixa capacidade de armazenamento de sementes de Caesalpinia echinata Lam. como consequência de processos oxidativos). O banco de sementes é uma das estratégias para a preservação de espécies ameaçadas de extinção, como Caesalpinia echinata Lam. Neste trabalho foram estudadas mudanças no consumo de O 2 e na liberação de CO 2 por sementes incubadas em diferentes temperaturas e teores de água, avaliando-se também a deterioração das sementes por meio dos testes de germinação e de tetrazólio. Os resultados demonstraram que os processos de deterioração que ocorrem nas sementes de C. echinata estão relacionados com a respiração e possivelmente com outros processos oxidativos, causando em curtos períodos a morte de tecidos embrionários e a perda da viabilidade das sementes. Essas características significam que a utilização do banco de sementes como alternativa para a conservação de Caesalpinia echinata depende do controle desses processos oxidativos. Palavras-chave: armazenamento, deterioração, pau-brasil, respiração de sementes 1. This work is part of the first Author's Master

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Short storability of Caesalpinia echinata Lam. seeds as a consequence of oxidative processes

Lamarca

Barbedo

2012

Hoehnea

Self Cite

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“…The fatty acids composition may vary among species due to the genetic load and climate conditions, and it may also vary among storage tissues. However, in oily seeds palmitic, oleic, and linoleic acids can reach higher amounts of up to 60% of the seeds mass (MELLO et al, 2010). According to Graham (2008), linoleic acid (linoleate) is an important precursor of other polyunsaturated fatty acids and is essential in mammal diets.…”

Section: Resultsmentioning

confidence: 99%

The morphological characterization of the dry seeds and reserve mobilization during germination inMorinda citrifoliaL.

Paula¹,

Sousa

Brito

et al. 2016

Revista Ciência Agronômica

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-Information about the morphology, chemical composition and reserve mobilization is important in understanding the establishment of native and exotic species. The purpose of this study was to describe the morphology, chemical composition, and mobilization of reserves during germination of noni (Morinda citrifolia L.). Biometric and morphological analyzes were performed with 100 randomly selected dried seeds. Other seeds were treated with sulfuric acid PA and soaked in Petri dishes. Collected seeds of five different times of germination were used for cytochemical and chemical analysis. For cytochemical analysis, the cuts of 5 m were submitted to dyes TB at pH 4.0; XP at pH 2.5, reaction of PAS and Sudan IV. The seeds were crushed for chemical analysis with lipids, proteins and soluble sugars extraction that were determined gravimetrically, by the Bradford method and the Antrona method, respectively. The fatty acid composition of the dry seed was determined by gas chromatography. Noni seeds are albuminous and have a thick seed coat, rich in lignin. Lipid and protein bodies were observed inside the endosperm cells, representing 43.50% and 9.15% respectively, while the reservoir of soluble sugars was less than 5%. Linoleic acid was the most prevalent with 68.1%. The lipids were mobilized during germination, suffering a reduction of up to 38% of its total. Proteins, as well as lipids decreased by 25.78% during the germination period observed. The main reserves of noni seeds are lipids and proteins that are mobilized during germination to provide energy and matter to the developing embryo and synthesis of more complex compounds.Key words: Cytochemical. Lipids. Fatty acids. Proteins. Germination.RESUMO -Informações sobre as características morfológicas, a composição química e mobilização de reservas são fundamentais na compreensão do estabelecimento de espécies nativas e exóticas. O objetivo deste trabalho foi descrever a morfologia, a composição química e a mobilização das reservas durante a germinação das sementes de noni (Morinda citrifolia L.). As análises morfológica e biométrica foram feitas com 100 sementes secas escolhidas aleatoriamente. Para as análises citoquímica cortes de 5 µm foram submetidos aos corantes AT em pH 4,0; XP em pH 2,5; reação do PAS e Sudan IV. As determinações químicas para lipídeos, proteínas e açúcares solúveis foram realizadas por gravimetria, pelo método de Bradford e pelo método da Antrona, respectivamente. A composição de ácidos graxos da semente seca também foi determinada por cromatografia gasosa. As sementes de noni são albuminosas e apresentam um tegumento espesso, rico em lignina. Foram observados corpos lipídicos e protéicos nas células do endosperma, representando 43,50% e 9,15%, respectivamente, enquanto que as reservas de açúcares solúveis foram inferiores a 5%. O ácido linoléico foi o mais predominante com 68,1%. Os lipídeos foram mobilizados, sofrendo redução de até 38% do seu total. Como também as proteínas sofreram redução de 25,78% durante o tempo de germinação ...

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“…Among the soluble sugars, the sucrose is one of the most accumulated in seeds and plays important role in the tolerance to cellular desiccation (Hincha et al, 2006 In studies of carbohydrates reserve composition, Mello et al (2010) have found that in seeds of Erythrina speciosa Andr., sucrose was not the main accumulated sugar. However, the same authors have observed high quantities of oligosaccharides of the raffinose (C 18 H 32 O 16 ) family, associated to tolerance to desiccation.…”

Section: Revista Brasileira De Sementes Vol 34 Nº 4 P 580 -588 2012mentioning

confidence: 99%

Mobilization of reserves and germination of seeds of Erythrina velutina Willd. (Leguminosae - Papilionoideae) under different osmotic potentials

Reis

Dantas

Pelacani³

2012

Rev. bras. sementes

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-Some environmental factors, including water availability, may influence seed germination. This study investigated the germination of E. velutina seeds submitted to different osmotic potentials and mobilization of reserves during water-stress. Scarified seeds were arranged in paper rolls and soaked in solutions of Polyethylene Glycol (PEG) prepared in osmotic potentials 0.0, -0.2, -0.4, -0.6, and -0.8 MPa and kept into a seed germinator, at 25 °C, and 12/12 h photoperiod (L/D), during 10 days. The percentage, mean time, mean speed, germination speed index; as well as the germination uniformity coefficient were assessed. During germination process the total soluble sugars, reducing sugars, soluble protein, and total amino acids were quantified in the cotyledon, hypocotyl and radicle of soaked seeds and cotyledons of quiescent seeds (control). There was influence of osmotic potential on E. velutina seed germination. The germination percentage remained at high levels until -0.6 MPa and above this osmotic potential there has been no germination. The mobilization of stored reserves of carbon and nitrogen in E. velutina seeds was also influenced by water-stress. There was sensitiveness between -0.2 and -0.6 MPa; however, the degradation and the mobilization of reserves was slower when the osmotic potential decreased.Index terms: PEG 6000, water-stress, carbon of storage, soluble proteins, "mulungu", "caatinga".Mobilização de reservas e germinação de sementes de Erythrina velutina Willd. (Leguminosae -Papilionoideae) sob diferentes potenciais osmóticos RESUMO -Alguns fatores ambientais podem influenciar a germinação, dentre eles a disponibilidade hídrica para as sementes. Este estudo investigou a germinação e a mobilização de reservas de sementes de E. velutina, submetidas a diferentes potenciais osmóticos. As sementes escarificadas foram embebidas em soluções de PEG preparadas em potenciais osmóticos de 0,0, -0,2, -0,4, -0,6 e -0,8 MPa, dispostas em rolos de papel e mantidas em germinador, a 25 °C e fotoperíodo de 12/12 h (L/E), durante 10 dias. Foi avaliado: porcentagem, tempo médio, velocidade média e índice de velocidade de germinação; e coeficiente de uniformidade de germinação. Durante a germinação foram quantificados: açúcares solúveis totais, açúcares redutores, proteínas solúveis e aminoácidos totais nos cotilédones, no hipocótilo e na radícula das sementes embebidas e nos cotilédones de sementes quiescentes (controle). Houve influência do potencial osmótico na germinação de sementes de mulungu. A porcentagem de germinação permaneceu em níveis elevados até -0,6 MPa; acima deste potencial não houve germinação. A mobilização de reservas de carbono e nitrogênio em sementes de E. velutina também foi influenciada pelo estresse hídrico. Houve sensibilidade entre -0,2 e -0,6 MPa, no entanto, a degradação e a mobilização de reservas foi mais lenta quando o potencial osmótico foi diminuído.Termos para indexação: PEG 6000, estresse hídrico, carbono de reserva, proteínas solúveis, mulungu, caatinga.

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Reserve carbohydrates and lipids from the seeds of four tropical tree species with different sensitivity to desiccation

Abstract: Considering the importance of water content for the conservation and storage of seeds

Cited by 39 publications

References 42 publications

Short storability of Caesalpinia echinata Lam. seeds as a consequence of oxidative processes

Short storability of Caesalpinia echinata Lam. seeds as a consequence of oxidative processes

The morphological characterization of the dry seeds and reserve mobilization during germination inMorinda citrifoliaL.

Mobilization of reserves and germination of seeds of Erythrina velutina Willd. (Leguminosae - Papilionoideae) under different osmotic potentials

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