Geochemical and isotopic characteristics of deep-seated saline groundwater provide valuable insight into the origin and evolving composition, water-rock interaction, and mixing potential of fossil brines. Such information may yield insight into intra-and interbasinal brine movement and relationships between brine evolution and regional groundwater flow systems. This investigation reports on the d 18 O and dD composition and activity values, 87 Sr/ 86 Sr ratios and Sr concentrations, and major ion concentrations of the Cambrian-hosted brines of the Mt. Simon Sandstone and Ironton-Galesville Formation and discusses the evolution of these brines as they relate to other intracontinental brines. Brines in the Illinois Basin are dominated by Na-Ca-Cl-type chemistry. The Mt. Simon and overlying Ironton-Galesville brines exhibit total dissolved solids concentrations of $195,000 mg/L and $66,270 mg/L, respectively. The dD of brine composition of the Mt. Simon ranges from À34& to À22& (V-SMOW), and the Ironton-Galesville is $À53.2& (V-SMOW). The d 18 O composition of the Mt. Simon brine ranges from À5.0& to À2.8& (V-SMOW), and the Ironton-Galesville brine is $À6.9& (V-SMOW). The 87 Sr/ 86 Sr values in the Mt. Simon brine range from 0.7110 to 0.7116. The less radiogenic Ironton-Galesville brine has an average 87 Sr/ 86 Sr value of 0.7107. Evaluation of d 18 O and dD composition and activities and 87 Sr/ 86 Sr ratios suggests that the Mt. Simon brine is likely connate seawater and recirculating deep-seated brines that have been diluted with meteoric water and influenced by the dissolution of evaporites with a minimal halite contribution based on Cl/Br ratios. The IrontonGalesville brine is also likely originally connate seawater that mixed with other brines and meteoric waters, including possibly Pleistocene glacial recharge. The Ca-excess vs. Na-deficiency comparison with the Basinal Fluid Line suggests the Mt. Simon and Ironton-Galesville brines have been influenced by the effects of albitization and plot very close to the Basinal Fluid Line. These Cambrian-hosted brines appear to have a different albitization history than other regional basin brines and a strong component of seawater. The Ironton-Galesville brine appears more geochemically associated with other Illinois Basin brines than the Mt. Simon brine which appears more geochemically conservative. Comparisons with other extrabasinal North American brines suggest that the Michigan basin brines are geochemically most similar to the Mt. Simon brines with the exception of the influence from carbonates in the Michigan Basin. Analyses of 87 Sr/ 86 Sr values in the Mt. Simon brine suggest that brine Sr has isotopically equilibrated with clay minerals in the Lower Mt. Simon and underlying bedrock formations and not with whole rock suggesting the influence of recirculating brines from the crystalline basement. Overall, the geochemistry of these Cambrian-hosted brines suggests an evolution from original seawater-like compositions. This investigation shows that intracratonic b...