Residual antigenicity of hypoallergenic infant formulas and the occurrence of milk-specific IgE antibodies in patients with clinical allergy

Beresteijn, E.C.H. van; Meijer, R. J. G. M.; Schmidt, Daniel

doi:10.1016/s0091-6749(95)70056-0

Cited by 63 publications

(34 citation statements)

References 36 publications

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“…An inhibition ELISA was carried out as described by Van Beresteijn et al (1995). Single wells of polystyrene microtiter plates (Greiner, Alphen a/d Rijn, The Netherlands) were coated with 135 mL of b-lactoglobulin (15 mg mL À1 in 0.05 mol L À1 sodium bicarbonate buffer, pH 9.6) and were incubated for 1.5 h at room temperature and then, overnight at 4 1C.…”

Section: Inhibition Elisa Testmentioning

confidence: 99%

“…However, residual allergenicity has been reported in several of these commercial preparations (Calvo & Gomez, 2002;Restani et al, 1995;Van Beresteijn, Meijer, & Schmidt, 1995), which could be due to inaccessibility of some epitopes to proteases even in the denatured protein. High pressure (HP) could be a valuable alternative to heat treatment to reduce the antigenicity of milk proteins, since several studies about the enhancement of the enzymatic proteolysis under HP have been reported.…”

Section: Introductionmentioning

confidence: 99%

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High pressure can reduce the antigenicity of bovine whey protein hydrolysates

Peñas¹,

Snel

Floris

et al. 2006

International Dairy Journal

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Section: Inhibition Elisa Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High pressure can reduce the antigenicity of bovine whey protein hydrolysates

Peñas¹,

Snel

Floris

et al. 2006

International Dairy Journal

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“…A peptide of 10 kDa or less is considered adequate to avoid this response; however there is evidence to suggest that the size needed may actually be smaller than three to five kDa [1]. Hydrolyzed diets are used in companion animals to diagnose and treat cutaneous and gastrointestinal adverse food reactions (AFR) [2].…”

Section: Introductionmentioning

confidence: 99%

A descriptive pilot study of cytokine production following stimulation of ex-vivo whole blood with commercial therapeutic feline hydrolyzed diets in individual healthy immunotolerant cats

et al. 2017

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Background: Hydrolyzed diets are used in companion animals for the diagnosis and treatment of adverse food reaction. Similarly, hydrolyzed formulas are used in human infants with severe inflammatory bowel disease or milk allergy, and these must meet the standard of hypoallergenicity through rigorous testing. Unfortunately, no standards are currently applied to hydrolyzed veterinary therapeutic diets, and data for the immunogenicity of feline diets is also not available. Therefore, the main aim of this pilot study was to determine if ex-vivo whole blood stimulation assays could be used to characterize the cytokine response to hydrolyzed commercial diets in a small number of individual healthy immunotolerant cats. This approach has also been used to investigate cytokine production in response to cow milk protein in humans and currently similar studies do not exist in companion animals. Nine healthy cats previously eating the same basal diet were divided into groups and fed one of three hydrolyzed diets exclusively for 6 weeks. Heparinized whole blood was collected from each cat before and after the feeding trial. Ex-vivo whole blood stimulation assays were performed using crude extracts of the basal diet as a positive control, as this diet contained the same proteins present in the hydrolyzed diet but were intact, saline as a negative control, and each cat's respective hydrolyzed diet. Supernatants were collected and analyzed for tumor necrosis factor-alpha, interleukin-10 (IL-10), and interleukin-4 using enzyme-linked immunosorbant assay. Results: Seven cats produced detectable amounts of the anti-inflammatory cytokine IL-10 upon stimulation with the basal diet. Two cats produced detectable amounts of IL-10 upon stimulation with a hydrolyzed soy-based diet and one cat produced a detectable amount of IL-10 upon stimulation with a hydrolyzed chicken-based diet (>125 pg/mL). Conclusions: Results from this pilot study suggest that in some healthy immunotolerant cats, some hydrolyzed diets may elicit a similar cytokine response compared to their basal diet, which contained the same proteins intact. Therefore, animals may be able to recognize and react to some hydrolyzed forms of tolerated proteins, and may also suggest IL-10 as a target for investigation as a potential marker for dietary tolerance in cats, however further studies would be necessary to corroborate this. Further studies are also needed to determine if this would also be the same in immunologically naïve, sensitized and clinically hypersensitized cats.

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“…In the case of partly hydrolyzed proteins, intact and partly hydrolyzed proteins are visible bands along an electrophoretic pattern of the products. Two commercial formula preparations with DH values of 6.3 and 1.3% contained some intact β-Lg and peptides with an Mr between 6000 and 8000 Da [192]. Using gel permeation chromatography, quantitative results on peptides with an Mr larger than 10 kDa were obtained [192].…”

Section: Reduction Of Allergenicity Of Milk Proteins By Hydrolysismentioning

confidence: 99%

“…Two commercial formula preparations with DH values of 6.3 and 1.3% contained some intact β-Lg and peptides with an Mr between 6000 and 8000 Da [192]. Using gel permeation chromatography, quantitative results on peptides with an Mr larger than 10 kDa were obtained [192]. Regardless of the technique used, descriptive information was obtained on either the molecular mass or the origin of the peptides.…”

Section: Reduction Of Allergenicity Of Milk Proteins By Hydrolysismentioning

confidence: 99%