2017
DOI: 10.1016/j.rbmo.2017.05.016
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Residual ethylene glycol and dimethyl sulphoxide concentration in human ovarian tissue during warming/thawing steps following cryopreservation

Abstract: There have been 60 births after transplantation of cryopreserved ovarian tissue: 58 using the slow freezing method, and two using the vitrification method. DMSO and EG are widely used as cryoprotectants. However DMSO is a known epimutagen, and EG has been reported to be toxic in high concentrations. In this study, we measured residual DMSO and EG in ovarian tissue after vitrification and slow freezing. Cryoprotectants remained at a high concentration in the vitrified/warmed ovarian tissue just before transplan… Show more

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Cited by 29 publications
(16 citation statements)
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“…However, we demonstrated in the present study that such combination is not the ideal choice for the agouti ovarian tissue, as it provokes a significant decrease on PFs viability. The presence of DMSO in media could result in histone modifications, which probably affects the definition of cellular properties (Nakamura et al, ), thus impairing viability of sensitive cells or tissues. Moreover, it is pointed that samples exposed to some CPAs combinations can remain at high concentrations (DMSO: 9.8 mg/g, EG: 9.8 mg/g) in the tissues, even after heating (Nakamura et al, ), which could impair the viability of some tissues as evident for the agouti ovary.…”
Section: Discussionmentioning
confidence: 99%
“…However, we demonstrated in the present study that such combination is not the ideal choice for the agouti ovarian tissue, as it provokes a significant decrease on PFs viability. The presence of DMSO in media could result in histone modifications, which probably affects the definition of cellular properties (Nakamura et al, ), thus impairing viability of sensitive cells or tissues. Moreover, it is pointed that samples exposed to some CPAs combinations can remain at high concentrations (DMSO: 9.8 mg/g, EG: 9.8 mg/g) in the tissues, even after heating (Nakamura et al, ), which could impair the viability of some tissues as evident for the agouti ovary.…”
Section: Discussionmentioning
confidence: 99%
“…Obata et al and Nakamura et al reported the residual cryoprotectants (around 30 mg/g in Ova Cryo Kit [46]) and around 10 mg/g DMSO and 10 mg/g EG in cryotissue [45] in ovarian tissue just before transplantation into human body [47,48]. Iwanani et al [49], Larman et al [50], and Cordeiro et al [51] reported cryoprotectant toxicity from gene expression profiling.…”
Section: Discussionmentioning
confidence: 99%
“…Although live births have been achieved by vitrification and the auto‐transplantation of ovarian tissue that has been harvested from patients with primary ovarian insufficiency, there has been no report of pregnancies being achieved by vitrification and the auto‐transplantation of ovarian tissue in oncofertility. One study found that the residual concentration of cryoprotectants after thawing was significantly higher with the Cryotissue method than with slow freezing . The clinical efficiency of ovarian tissue cryopreservation by vitrification or slow freezing cannot be compared until the pregnancy outcomes for auto‐transplantation by vitrification are known, which probably will take several years.…”
Section: Ovarian Tissue Cryopreservation: Practice and Problemsmentioning
confidence: 99%
“…One study found that the residual concentration of cryoprotectants after thawing was significantly higher with the Cryotissue method than with slow freezing. 62 The clinical efficiency of ovarian tissue cryopreservation by vitrification or slow freezing cannot be compared until the pregnancy outcomes for auto-transplantation by vitrification are known, which probably will take several years.…”
Section: Slow Freezing and Vitrificationmentioning
confidence: 99%