Residue depletion of toltrazuril and its metabolites in egg after an authorised application in hens

Dendougui-Tadjine, Ferial; Pröhl, Rüdiger; Spies, Diana; Hamann, Frank; Schulz, F; Terhalle, W.; Lange, Nikola; Scheid, Stefan; Polzer, J.

doi:10.1016/j.foodcont.2022.109596

Cited by 2 publications

(1 citation statement)

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“…For muscle, the following maximum residue limits (MRLs): 500 μg/kg for DIZ, 100 μg/kg for TOZ, and 200 μg/kg for NICA were set [ 9 ]. TOZ is absorbed via the gastrointestinal tract and metabolized into the biologically active forms toltrazuril sulphoxide (TZSO) and toltrazuril sulphone (TZS) [ 10 ]. Over the past few decades, global attention has increasingly focused on food safety as a matter of paramount importance.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Nicarbazin, Diclazuril, Toltrazuril, and Its Two Metabolites in Chicken Muscle and Eggs by In-Syringe Dispersive Solid-Phase Filter Clean-Up Followed by Liquid Chromatography–Tandem Mass Spectrometry

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et al. 2024

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Nicarbazin (NICA) and triazine anticoccidial drugs (diclazuril (DIZ) and toltrazuril (TOZ)) are the primary strategy for preventing and treating coccidiosis. To prevent the development of drug resistance and mitigate the potential chronic toxicity to humans resulting from prolonged exposure, a liquid chromatography–tandem mass spectrometry method with high reliability and sensitivity was developed to determine NICA, DIZ, TOZ, and its two metabolites in chicken muscle and eggs. Upon establishing the extraction conditions involving 10 mL of acetonitrile and 10 min of sonication, in-syringe dispersive solid-phase extraction with silica was performed in combination with n-hexane clean-up. The selection of isotope peaks of precursor ions and low-mass range scanning allowed the two transitions for the quantification of all compounds. The limits of detection for DIZ and NICA were both 0.1 μg/kg, and for TOZ and metabolites, they were 0.3 μg/kg; the limits of quantitation were 0.3 and 1 μg/kg, respectively. The linear range was 0.25–50 ng/mL with a correlation coefficient r > 0.999. The average recoveries at three spiking levels in muscle and eggs were 90.1–105.2% and 94.0–103.7% with the relative standard deviations of 3.0–8.1% and 3.1–14.4%, respectively. The precision, accuracy, and stability were evaluated by three quality control samples.

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Section: Introductionmentioning

confidence: 99%