Surfactant residues (Tween 80 Esters and Tween 80 free Polyols) were determined in Maryland tobacco treated with a fatty-alcohol-type sucker-control formulation. The levels of residues in leaves decreased throughout the growing and air-curing periods, but decreased the most during the first 24 hours after treatment. Factors affecting Tween 80 residue levels included dosage and location of leaves on the plant.