2005
DOI: 10.1016/j.jmb.2005.04.073
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Residues in the Conserved His Domain of Fruit Fly tRNase Z that Function in Catalysis are Not Involved in Substrate Recognition or Binding

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Cited by 30 publications
(59 citation statements)
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“…1A,B), are investigated herein, and the recently analyzed His domain (Motif II) ( Fig. 1C; Zareen et al 2005) is included to help establish context. Five eukaryotic tRNase Z L s (above) and three bacterial tRNase Z S s (below) are shown in the alignments.…”
Section: Homology Blocks On the Amino Side Of The His Domain (Motif Ii)mentioning
confidence: 99%
“…1A,B), are investigated herein, and the recently analyzed His domain (Motif II) ( Fig. 1C; Zareen et al 2005) is included to help establish context. Five eukaryotic tRNase Z L s (above) and three bacterial tRNase Z S s (below) are shown in the alignments.…”
Section: Homology Blocks On the Amino Side Of The His Domain (Motif Ii)mentioning
confidence: 99%
“…Gels were dried and exposed overnight using a storage phospor screen which was scanned with the Typhoon and analyzed using ImageQuant. 8 In this approximation of zero order kinetics, % product/minute of reaction (V/[S]) is proportional to V. Reaction velocities for variant and wild type pre-tRNA substrates were matched by adjusting enzyme concentrations.…”
Section: Methodsmentioning
confidence: 99%
“…The Motif II signature sequence, HxHxDH, contributes four of these residues (reviewed in ref. 8). Histidines in motifs III and V and aspartate in motif IV contribute coordination bonds to the metals and are also important for catalysis.…”
Section: Introductionmentioning
confidence: 99%
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“…The C-terminal half region of tRNase Z L has high similarity to the whole region of tRNase Z S and these regions contain a well-conserved histidine motif, which has been shown to be essential for the tRNase Z activity. 5,8 We have demonstrated in vitro that mammalian tRNase Z L can cleave any target RNA at any desired site by recognizing a pre-tRNA-like or micro-pre-tRNAlike complex formed between the target RNA and artificial small-guide RNA (sgRNA). [9][10][11][12] sgRNA is divided into four categories: 5 0 -half-tRNA, 10 14-nucleotide (nt) linear RNA, 13 RNA heptamer 11,12 and hook RNA.…”
Section: Introductionmentioning
confidence: 99%