Residues of deoxynivalenol (DON) in pig tissue after feeding mash or pellet diets containing low concentrations

Döll, Susanne; Dänicke, Sven; Valenta, Hana

doi:10.1002/mnfr.200700308

Cited by 35 publications

(12 citation statements)

References 14 publications

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“…For instance, in a recent study DON was detected in 98.7% of urine samples from adults in the United Kingdom [11]. In a recent study, pigs fed with low concentrations of DON showed toxin levels of up to 0.5 and 0.02 lM in bile and liver, respectively [15]. These levels are clearly below the 1 lM dose used in this study, which therefore may reflect worst case intake scenario rather than mean exposure.…”

Section: Discussionmentioning

confidence: 50%

Potential of deoxynivalenol to induce transcription factors in human hepatoma cells

Nielsen

Lippke

Didier

et al. 2009

Molecular Nutrition Food Res

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To assess the hepatotoxicity of deoxynivalenol (DON), human hepatoma cells (Hep-G2) were used as an in vitro model. After exposing Hep-G2 cells to low (1 mciroM) and high dose (10 mciroM), gene expression profiles were analysed by microarray. More than 5% of genes were up-regulated, most of them being involved in transcriptional regulation. By real-time RT-PCR, elevated expression of transcription factors, commonly induced by activation of MAPK-pathway, was demonstrated for Hep-G2 cells on mRNA and protein level. Further studies, involving U937 human monocytes, showed that effects of DON treatment on mRNA and protein level were concentration-dependent and cell-specific. An inverse relation was noticed for the level of DON induced expression of transcription factors (JUN, FOS, EGR1 and ATF3) and the susceptibility of the cell lines towards the mycotoxin. This is the first report giving evidence that on a molecular level the mild hepatotoxic effects of DON are probably caused by the induction of transcription factors which are known to be associated with injury-induced liver regeneration processes. With ATF3, a novel downstream target gene was identified in DON-related cell signalling suggesting a potential linkage between molecular action and biological effects like reduction of glycogen storage in liver tissue.

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Section: Discussionmentioning

confidence: 50%

Potential of deoxynivalenol to induce transcription factors in human hepatoma cells

Nielsen

Lippke

Didier

et al. 2009

Molecular Nutrition Food Res

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“…Using the obtained results, the carry-over factor was calculated as the quotient of toxin concentration in the tissue (µg/kg) by toxin concentration in the diet (µg/kg), included as the total administered doses in the defined term experiment, according to previous researchers [38,43,44]…”

Section: Resultsmentioning

confidence: 99%

“…In a similar study the concentrations of DON decreased from bile > kidney > serum > liver = muscle in an experiment, where pigs received diets containing 0%, 25% and 50% of contaminated wheat containing 2.5 mg DON/kg [43]. …”

Section: Resultsmentioning

confidence: 99%

Deoxynivalenol in the Gastrointestinal Tract of Immature Gilts under per os Toxin Application

Waśkiewicz

Beszterda

Kostecki

et al. 2014

Toxins

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Deoxynivalenol is also known as vomitoxin due to its impact on livestock through interference with animal growth and acceptance of feed. At the molecular level, deoxynivalenol disrupts normal cell function by inhibiting protein synthesis via binding to the ribosome and by activating critical cellular kinases involved in signal transduction related to proliferation, differentiation and apoptosis. Because of concerns related to deoxynivalenol, the United States FDA has instituted advisory levels of 5 µg/g for grain products for most animal feeds and 10 µg/g for grain products for cattle feed. The aim of the study was to determine the effect of low doses of deoxynivalenol applied per os on the presence of this mycotoxin in selected tissues of the alimentary canal of gilts. The study was performed on 39 animals divided into two groups (control, C; n = 21 and experimental, E; n = 18), of 20 kg body weight at the beginning of the experiment. Gilts received the toxin in doses of 12 µg/kg b.w./day (experimental group) or placebo (control group) over a period of 42 days. Three animals from two experimental groups were sacrificed on days 1, 7, 14, 21, 28, 35 and 42, excluding day 1 when only three control group animals were scarified. Tissues samples were prepared for high performance liquid chromatography (HPLC) analyses with the application of solid phase extraction (SPE). The results show that deoxynivalenol doses used in our study, even when applied for a short period, resulted in its presence in gastrointestinal tissues. The highest concentrations of deoxynivalenol reported in small intestine samples ranged from 7.2 (in the duodenum) to 18.6 ng/g (in the ileum) and in large intestine samples from 1.8 (in transverse the colon) to 23.0 ng/g (in the caecum). In liver tissues, the deoxynivalenol contents ranged from 6.7 to 8.8 ng/g.

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“…In addition, metabolite de-epoxy-DON was detected only in liver (0–2.4 ng/g) (Doll et al ., 2008). The maximum recalculating factor (the sum of the DON and de-epoxy-DON in tissues divided by concentration of DON administered in the diet) was 0.0043 for muscle 0.0064 for liver and 0.0319 in the kidney (Doll et al ., 2008). These factors are very similar to those of other studies performed on pigs, which were fed with a diet contaminated by DON (6.68 mg/kg) for 12 weeks.…”

Section: Trichothecenes and Deoxynivalenolmentioning

confidence: 99%

Deoxynivalenol and its toxicity

Šobrová

Adam²,

Vasatkova³

et al. 2010

Interdisciplinary Toxicology

418

293

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Deoxynivalenol (DON) is one of several mycotoxins produced by certain Fusarium species that frequently infect corn, wheat, oats, barley, rice, and other grains in the field or during storage. The exposure risk to human is directly through foods of plant origin (cereal grains) or indirectly through foods of animal origin (kidney, liver, milk, eggs). It has been detected in buckwheat, popcorn, sorgum, triticale, and other food products including flour, bread, breakfast cereals, noodles, infant foods, pancakes, malt and beer. DON affects animal and human health causing acute temporary nausea, vomiting, diarrhea, abdominal pain, headache, dizziness, and fever. This review briefly summarizes toxicities of this mycotoxin as well as effects on reproduction and their antagonistic and synergic actions.

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Residues of deoxynivalenol (DON) in pig tissue after feeding mash or pellet diets containing low concentrations

Cited by 35 publications

References 14 publications

Potential of deoxynivalenol to induce transcription factors in human hepatoma cells

Potential of deoxynivalenol to induce transcription factors in human hepatoma cells

Deoxynivalenol in the Gastrointestinal Tract of Immature Gilts under per os Toxin Application

Deoxynivalenol and its toxicity

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