Resin-Based Cement Applied to Enamel and Dentin Pre-Treated with Phytic Acid: An In Vitro Study

Abstract

Phytic acid (IP6) has the ability to etch tooth structure due to its acidity and chelating properties. This study aimed to evaluate the micro-tensile bond strength (μTBS) of resin-based cement to enamel and dentin using manufacturer-provided etchants, phosphoric acid (PA) for enamel and a mixture of 10% citric acid and 3% ferric chloride (CAF) for dentin and compare them to IP6. Enamel surfaces were etched for 30 s with either PA or IP6 (1%, 5% or 10%). Dentin surfaces were etched for 15 s with either CAF or 1% IP6. Pre-polymerized composite blocks were bonded to enamel and dentin surfaces. After 24 h, the specimens were sectioned into beams and subjected to μTBS testing. De-bonded specimens and smear layer removal were analyzed with a scanning electron microscope. Etching enamel with 1% and 5% IP6 resulted in μTBS values that are comparable to the control (PA) (p > 0.05), while 10% IP6 negatively affected the μTBS (p < 0.05). The μTBS to dentin etched with 1% IP6 was higher compared to the control (CAF) (p = 0.001). IP6 effectively removed enamel and dentin smear layer, and at specific concentrations, it may be a suitable alternative conditioner agent for bonding resin-based cement to enamel and dentin.

Effectiveness of Phytic Acid as an Etchant in Dentistry: A Systematic Review

“…Furthermore, compared to PA, 1% IP6 causes significantly less enamel surface erosion. 24 The hypothesis evaluated in this review was accepted because IP6 effectively etched dentin and eliminated the smear layer, resulting in excellent bond strength values with low nanoleakage, dentin dry mass loss, and collagen degra-dation. 13 Moreover, a cell line study with rat pulpal cells showed insignificant changes with usage of phytic acid, whereas PA significantly reduced their viability as it damages the DNA in human lymphocytes.…”

Phytic acid effect on periodontal ligament fibroblast: An in-vitro study

“…IP6 effects on starch digestibility, hormones, insulin signaling, and glucose metabolism have been proposed as possible pathways [ 16 , 17 ]. The attentiveness to IP6 and some of its applications in dentistry have been described several decades ago, however, the interest declined until recently revived by exploring further properties that are applicable to the dental field [ 18 , 19 ]. The anti-plaque and anti-calculus functions of IP6 prompted its incorporation within oral care products to act locally in the oral cavity to prevent the progression of periodontal disease [ 20 ].…”