Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro

Issa, Yasmin; Watts, David C.; Brunton, Paul; Waters, Catherine; Duxbury, A J

doi:10.1016/s0109-5641(03)00053-8

Cited by 193 publications

(148 citation statements)

References 38 publications

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“…A pronounced cytotoxic effect of dental materials on cell cultures of oral cells has been reported. 41,42 Another cause of lesions related to dental restorations may be immunological or toxic reactions to plaque accumulations on the surfaces of the restorations. Such lesions may disappear after improved oral hygiene.…”

Section: Discussionmentioning

confidence: 99%

Oral lichenoid lesions related to dental restorative materials

Issa

Duxbury²,

Brunton³

2005

Br Dent J

Self Cite

106

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Section: Discussionmentioning

confidence: 99%

Oral lichenoid lesions related to dental restorative materials

Issa

Duxbury²,

Brunton³

2005

Br Dent J

Self Cite

106

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“…It is the most commonly used test to evaluate dental material cytotoxicity because it is an easy, fast and inexpensive test [82,90,91]. The MTT assay was described by Mossmann (1983) in order to detect the viability and proliferation of mammalian cells [92].…”

Section: Cytotoxicitymentioning

confidence: 99%

“…The optical absorbance is directly proportional to the amount of dissolved formazan and the number of viable cells [93]. Issa et al [90] tested different dental monomers and their toxic effect on human gingival fibroblast using two different cytotoxicity tests. MTT was used to measure fibroblasts metabolism and the cytosolic enzyme lactate dehydrogenase (LDH) was used as a measure of cell integrity.…”

Section: Cytotoxicitymentioning

confidence: 99%

Reinforcement of flowable dental composites with titanium dioxide nanotubes

et al. 2016

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“…Colorimetric assays, which involve specific colorants and light adsorption measurements, remain the most commonly used, due to their relative simplicity and low cost [22][23][24][25][26] . Microscopy analysis by light contrast frequently consumes more time and incurs higher costs.…”

Section: Introductionmentioning

confidence: 99%

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Attik¹,

Gritsch²,

Colon³

et al. 2014

JoVE

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It is generally accepted that in vitro cell material interaction is a useful criterion in the evaluation of dental material biocompatibility. The objective of this study was to use 3D CLSM time lapse confocal imaging to assess the in vitro biocompatibility of dental composites. This method provides an accurate and sensitive indication of viable cell rate in contact with dental composite extracts. The ELS extra low shrinkage, a dental composite used for direct restoration, has been taken as example. In vitro assessment was performed on cultured primary human gingival fibroblast cells using Live/Dead staining. Images were obtained with the FV10i confocal biological inverted system and analyzed with the FV10-ASW 3.1 Software. Image analysis showed a very slight cytotoxicity in the presence of the tested composite after 5 hours of time lapse. A slight decrease of cell viability was shown in contact with the tested composite extracts compared to control cells. The findings highlighted the use of 3D CLSM time lapse imaging as a sensitive method to qualitatively and quantitatively evaluate the biocompatibility behavior of dental composites.

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Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro

Cited by 193 publications

References 38 publications

Oral lichenoid lesions related to dental restorative materials

Oral lichenoid lesions related to dental restorative materials

Reinforcement of flowable dental composites with titanium dioxide nanotubes

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

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