Resistance and virulence factors of<i>Escherichia coli</i>isolated from chicken

Pavlíčková, Silvie; Doležalová, Magda; Holko, Ivan

doi:10.1080/03601234.2015.1011959

Cited by 11 publications

(7 citation statements)

References 29 publications

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“…Such resistance phenotypes are generally attributed to the misuse of antibiotics, which have increased invulnerability to hamper the treatment of infection, and indirectly increase the rate of mortality. Antibiotic use and resistance presents a real dilemma for developed and developing countries (Fortini et al 2011; Pavlickova et al 2015; Wellington et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy

et al. 2019

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Bacteriophages can be used successfully to treat pathogenic bacteria in the food chain including zoonotic pathogens that colonize the intestines of farm animals. However, harsh gastric conditions of low pH and digestive enzyme activities affect phage viability, and accordingly reduce their effectiveness. We report the development of a natural protective barrier suitable for oral administration to farm animals that confers acid stability before functional release of bead-encapsulated phages. Escherichia coli bacteriophage ZSEC5 is rendered inactive at pH 2.0 but encapsulation in chitosan–alginate bead with a honey and gelatin matrix limited titer reductions to 1 log 10 PFU mL −1 . The encapsulated phage titers were stable upon storage in water but achieved near complete release over 4–5 h in a simulated intestinal solution (0.1% bile salt, 0.4% pancreatin, 50 mM KH 2 PO 4 pH 7.5) at 37 °C. Exposure of E. coli O157:H7 to the bead-encapsulated phage preparations produced a delayed response, reaching a maximal reductions of 4.2 to 4.8 log 10 CFU mL −1 after 10 h at 37 °C under simulated intestinal conditions compared to a maximal reduction of 5.1 log 10 CFU mL −1 at 3 h for free phage applied at MOI = 1. Bead-encapsulation is a promising reliable and cost-effective method for the functional delivery of bacteriophage targeting intestinal bacteria of farm animals.

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Section: Introductionmentioning

confidence: 99%

Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy

et al. 2019

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“…For instance, the rate of ciprofloxacin resistance in clinical E. coli isolates has increased from 4.3% to 16.7% between 1998 and 2013 in southeast Austria. Such resistance is attributed to the misuse of antibiotics and will ultimately increase the cost of treatment, prolong the illness, and increase the rate of mortality (Van et al 2007;Bhowmick et al 2011;Fortini et al 2011;Pavlickova et al 2015). Unfortunately, more than half a million people worldwide die each year from antibiotic-resistant bacterial infections (Davies et al 2013).…”

Section: Antibiotic-associated Intestinal Dysbiosismentioning

confidence: 99%

Bacteriophages: the possible solution to treat infections caused by pathogenic bacteria

El‐Shibiny

El-Sahhar

2017

Can. J. Microbiol.

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Since their discovery in 1915, bacteriophages have been used to treat bacterial infections in animals and humans because of their unique ability to infect their specific bacterial hosts without affecting other bacterial populations. The research carried out in this field throughout the 20th century, largely in Georgia, part of USSR and Poland, led to the establishment of phage therapy protocols. However, the discovery of penicillin and sulfonamide antibiotics in the Western World during the 1930s was a setback in the advancement of phage therapy. The misuse of antibiotics has reduced their efficacy in controlling pathogens and has led to an increase in the number of antibiotic-resistant bacteria. As an alternative to antibiotics, bacteriophages have become a topic of interest with the emergence of multidrug-resistant bacteria, which are a threat to public health. Recent studies have indicated that bacteriophages can be used indirectly to detect pathogenic bacteria or directly as biocontrol agents. Moreover, they can be used to develop new molecules for clinical applications, vaccine production, drug design, and in the nanomedicine field via phage display.Key words: bacteriophages, antibiotics, biocontrol, infection, pathogenesis.Résumé : Les bactériophages ont été utilisés pour traiter des infections bactériennes chez l'animal et l'humain depuis leur découverte en 1915, à cause de leur capacité unique d'infecter leurs hôtes bactériens spécifiques, sans affecter les autres populations bactériennes. La recherche réalisée dans ce domaine au cours du vingtième siècle, principalement en Géorgie, dans une partie de l'URSS et en Pologne, a mené à l'établissement de protocoles thérapeutiques à partir de phages. Cependant, la découverte de la pénicilline et des antibiotiques sulfamidés au cours des années 1930 a retardé l'avancement de la thérapie par les phages. Le mauvais usage des antibiotiques depuis leur découverte a résulté en une augmentation du nombre de bactéries résistantes aux antibiotiques et une réduction de leur efficacité à contrôler d'importants pathogènes. Les bactériophages sont devenus un sujet d'intérêt comme alternative aux antibiotiques avec l'émergence de bactéries multirésistantes qui constituent une menace à la santé publique. Des études récentes ont indiqué que les bactériophages peuvent être utilisés indirectement pour détecter les bactéries pathogènes, ou directement, comme agents de contrôle biologique. De plus, ils peuvent être utilisés pour développer de nouvelles molécules à des fins d'applications cliniques, de production de vaccins, dans la conception de médicaments et dans le domaine de la nanomédecine, par l'exposition sur phage. [Traduit par la Rédaction]

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“…Among bacterial diseases, the proportion of animals dead due to colibacillosis was the highest, followed by clostridiosis. Both are important infectious diseases in chicks, and affect poultry worldwide [ 29 , 30 , 31 ], with several cases of C. perfringens infection causing necrotizing enteritis or necrotic hepatitis reported in captive capercaillies [ 32 ]. The etiological agents— E. coli and C. perfringens —use several virulence strategies to achieve tissue invasion such as metabolic enzymes, adhesion molecules, or tissue-degrading toxins [ 31 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Mortality Causes in Captive Cantabrian capercaillie (Tetrao urogallus cantabricus) in Spain

García‐Rodríguez

Herrero-García

García

et al. 2023

Animals

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The Cantabrian capercaillie (Tetrao urogallus cantabricus) is one of the most severely threatened subspecies of capercaillie. Its current population range is restricted to a small area of the Cantabrian Mountains (northwestern Spain), with only around 200 individuals remaining. As part of the national strategy for the conservation of the subspecies, the Cantabrian capercaillie Captive Breeding Center of Sobrescobio opened in 2009. Here, we use the information provided by the necropsies performed in this facility on 29 individuals (11 males, 13 females and 5 undetermined; 16 chicks and 13 adults) in order to describe the main mortality causes of captive-bred Cantabrian capercaillies. After necropsy, tissue samples were taken for evaluation using standard methods in histology and microbiology. The majority of the captive animals (18/29, 62.07%) died due to infectious diseases, mainly due to Escherichia coli, Clostridium perfringens, or Aspergillus fumigatus infection. The remaining 11 animals died due to stress-related processes (i.e., rupture of the heart apex and cardiomyopathy or neurogenic shock) (8/29, 27.59%), duodenal obstruction and coelomitis (1/29, 3.45%), perforation of the proventriculus and heart with a briar branch (1/29, 3.45%) or euthanasia due to a valgus leg deformity that prevented proper animal welfare (1/29, 3.45%). Young animals (i.e., younger than 2 months) died mainly due to infectious diseases (14/16, 87.5%), while stress-related causes were responsible for most adult deaths (7/13, 53.85%). We additionally report that two free-ranging adult males died due to exertional myopathy. This study provides relevant information for reducing mortality in captive capercaillies and improving both living conditions in captivity and the adaptation of these animals to the wild.

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Resistance and virulence factors ofEscherichia coliisolated from chicken

Cited by 11 publications

References 29 publications

Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy

Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy

Bacteriophages: the possible solution to treat infections caused by pathogenic bacteria

Mortality Causes in Captive Cantabrian capercaillie (Tetrao urogallus cantabricus) in Spain

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