Resistance Changes in Lipid Bilayers: Immunological Applications

Barfort, Per; Arquilla, Edward R.; Vogelhut, P. O.

doi:10.1126/science.160.3832.1119

Cited by 50 publications

(9 citation statements)

References 10 publications

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“…This suggests that the process of glucose permeation of the phosphatidyl choline bimolecular membrane, including that accelerated by the presence of insulin, occurs without a net flux of ions through the bilayer. These findings are compatible with those in the literature (Wood etaL, 1968;Jung, 1971)in which no change in the electrical resistance of lipid bimolecular membranes occurred during glucose permeation, and none (Hanai, Haydon &Taylor, 1965;Barfort, Arquilla & Vogelhut, 1968) when insulin was added.…”

Section: Discussionsupporting

confidence: 93%

The effect of insulin on the permeability of phosphatidyl choline bimolecular membranes to glucose

Kafka

1974

J. Membrain Biol.

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Section: Discussionsupporting

confidence: 93%

The effect of insulin on the permeability of phosphatidyl choline bimolecular membranes to glucose

Kafka

1974

J. Membrain Biol.

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“…These observations are reminiscent of the inhibition of the excitability phenomena induced by EIM (excitability-inducing material) on BLM by protease addition to the compartment opposite to that where EIM is present (cf. Mueller & Rudin, 1969b) and the antigen-antibody reaction occurring when the components are present on opposite sides of the membrane, provided complement is supplied (Barfort, Arquilla & Vogelhust, 1968). This trypsin-sensitivity of the electrical changes induced by Ps + on anionic BLM, taken together with the expansion of stearic acid monolayers in the presence of Ps + (Shah, 1969(Shah, , 1970) and the nuclear magnetic resonance evidence of the Ps+-induced immobilization of the fatty acid chains of phosphatidylserine vesicles (Hammes & Schullery, 1970) indicates that the "polypeptide phase" extends across the lipid film and suggests that while the initial force determining the interaction is predominantly electrostatic, the overall complex formation and membrane reassembly involves important hydrophobic interactions between the lipid and polypeptide components.…”

Section: Discussionmentioning

confidence: 99%

Lipid-polypeptide interactions in bilayer lipid membranes

Montal

1972

J. Membrain Biol.

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The modifications of the electrical properties of bilayer lipid membranes (BLM) composed of cholesterol and an ionic surfactant upon interaction with charged polypeptides were studied. The addition of 10(-8) M polylysine (Ps(+)) to one side of anionic cholesterol dodecylphosphate BLM increases the specific membrane conductance over 1000-fold (from 10(-8) to 10(-5) mho/cm(2)) and develops a cationic transmembrane potential larger than 50 mV. This potential is reverted by addition of polyanions such as RNA, polyglutamic or polyadenilic acid to the same side on which Ps(+) is present, by addition of Ps(+) to the opposite side, or by addition of trypsin to either side. Both conductance and potential changes are hindered by increasing the ionic strength or by raising the pH of the bathing medium, disappearing above pH 11.5 where it is known that Ps(+) folds into an α-helix. The interaction of polyglutamic acid (PGA) with a cationic cholesterol-hexadecyltrimethylammonium bromide BLM results in increased membrane conductance and development of an anionic transmembrane potential which is reverted by addition of polycations to the same aqueous phase where PGA is present. Addition of either Ps(+) or PGA to one or both sides of a neutral BLM composed of 7-dehydrocholesterol induces no significant change. The observations suggest the formation of a lipid polymer membrane resultant from the interaction, predominantly electrostatic, of the isolated components. The implications of these results are discussed in terms of the current models of membrane structure.

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“…A similar effect has been reported subsequently by two other laboratories (20,42). Recently Barfort, Arquilla & Vogelhut (114) observed that if antibody and complement are on one side of a sphingomyelin-a tocopherol bilayer and the homologous antigen is added to the other side, the bilayer resistance decreases.…”

Section: Modifications Of the Electrical Properties Of Bilayers--thementioning

confidence: 99%