Resistance Evaluation of Commmercial Tomato Cultivars against Tomato yellow leaf curl virus

Abstract

Tomato yellow leaf curl virus (TYLCV) is a viral disease causing severe economic losses on tomato. Practical prevention of the TYLCV disease is to control tabacco whitefly (Bemisia tabaci) or to cultivate TYLCV-resistant tomato cultivars, because no agrochemical products are available to control TYLCV. In this study, TYLCV resistance of the commercial tomato cultivars were evaluated using the DNA markers tightly linked to TYLCV resistance genes Ty-1 and Ty-3 and infection with the TYLCV clones mediated by Agrobacterium. In marker genotyping, resistance alleles were detected from 4 oval type tomato cultivars (Titichal, TY tinny, TY saengsaeng II, TY sense Q). Four cheery type cultiavrs (TY endorphin, TY smartsama, Tiara TY, Olleh TY) and 6 round type cultivars (TY kingdom, TY ace, TY homerun, TY altorang, Dotaerang TY winner, Styx TY). The seedling bioassay indicated that tomato cultivars of the oval type and cherry type showed consistancy in marker genotype and phenotype while slight disease symptom was observed from some round type cultivras (TY ace, TY homerun, Styx TY) with resistance marker genotype. For fruit yields, TY tinny was greater than its control cultivar Titichal in oval types, TY smartsama was greater than its control Smile in cherry type, and TY ace and TY kingdom were greater than their control Dabok. These cutliavrs can be a good choice for high-yielding TYLCVresistant tomato cultivars.

Evaluation of Grafted Tomatoes with Different Levels of Resistance of Rootstocks to TYLCV by Analyzing the Growth Characteristics, Leaf-macronutrient Content, and Chlorophyll Fluorescence

“…매개충인 담배가루이는 세대 기간이 짧고 증식률이 높아 살충제 살포를 하면 갈수록 약제 저항성이 높아져 화학적 방제가 어렵다. 물리적 방제로 방충망을 설치하여 유입 자체를 차단하는 방법이 있으나 기존 온실에 방충망을 설치하는 투자 비용이 높을뿐더러 환기율이 떨어져 고온기 온실 내 온도 제어가 어렵기 때문에 방충망을 설치하지 않 는 실정이다 (Ko et al, 2016). 이렇게 TYLCV는 화학적 또는 물리적 방제가 어려워 바이러스 내병성 토마토 품종을 사용하는 것이 최선의 방법이나 TYLCV 내병성 품종이어도 온실 내 매개충의 밀도가 높으면 바이러스 발병 확률이 높아져 (Kim and Kim, 2016) 저항성을 더 높일 수 있는 방안을 찾는 것이 필요하다.…”