Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

İnak, Emre; Alpkent, Yasin Nazım; Çobanoğlu, Sultan; Dermauw, Wannes; Leeuwen, Thomas Van

doi:10.1007/s10493-019-00398-w

Cited by 38 publications

(20 citation statements)

References 65 publications

(97 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Abamectin has been used extensively for spider mite control, and over the years various levels of abamectin resistance have been detected in T. urticae populations worldwide. 31,33,36,45,60 The molecular mechanism underlying abamectin resistance in T. urticae have been investigated in a number of strains and both enhanced metabolic detoxification as well as target-site resistance mutations were shown to be at play. In particular, geneexpression analysis and functional characterization have pointed towards the involvement of cytochrome P450s (P450s), glutathione-S-transferases (GSTs) and uridine diphosphate (UDP) -glycosyltransferases (UGTs).…”

Section: Discussionmentioning

confidence: 99%

“…The abamectin resistant strain IT6 has been previously described and of all five GluCl genes only GluCl3 carried an I321T mutation, 36 while the TM2/TM3 region of Rdl1 / 2 / 3 was screened in this study (see 2.2) The abamectin resistant strain TR2 (corresponding to sample number 2 in İnak et al . 2019) has been previously described and lacks the previously documented abamectin resistance mutations, G314D in GluCl1 and G326E in GluCl3 45 . The TM3 region of GluCl2 / 4 / 5 , I321T mutation in GluCl3 and TM2/TM3 region Rdl1 / 2 / 3 of TR2 were screened in this study (see 2.2).…”

Section: Methodsmentioning

confidence: 99%

“…For each replicate, about 20–30 young adult female mites were transferred to 9‐cm 2 bean leaf disks and sprayed with serial pesticide dilutions at 1 bar pressure. For IT6 and congenic lines (2.4), a Cornelis spray tower was used for spraying leaf disks and 0.8 mL of pesticide dilution was applied to obtain a 1.5 ± 0.05 mg aqueous acaricide deposit cm −2 45,46,48 . For TR2 and congenic lines (2.4), a Potter spray tower (Burkard Scientific Ltd, Uxbridge, UK) was used for spraying and 2 mL of acaricide dilution was applied to obtain a 1.95 ± 0.05 mg aqueous acaricide deposit cm −2 .…”

Section: Methodsmentioning

confidence: 99%

“…2) The abamectin resistant strain TR2 (corresponding to sample number 2 in İnak et al 2019) has been previously described and lacks the previously documented abamectin resistance mutations, G314D in GluCl1 and G326E in GluCl3. 45 The TM3 region of GluCl2/4/5, I321T mutation in GluCl3 and TM2/TM3 region Rdl1/ 2/3 of TR2 were screened in this study (see 2.2). In our previous study, the strains IT1, IT5 and ES1 were screened for the presence of mutations in TM3 of GluCl1/2/3/4/5 36 while the TM2/TM3 regions of Rdl1/2/3 of these strains were screened in this study (see 2.2).…”

Section: Mite Strainsmentioning

confidence: 99%

See 3 more Smart Citations

Untangling a Gordian knot: the role of a GluCl3 I321T mutation in abamectin resistance in Tetranychus urticae

Xue

Mermans

Papapostolou

et al. 2020

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND The cys‐loop ligand‐gated ion channels, including the glutamate‐gated chloride channel (GluCl) and GABA‐gated chloride channel (Rdl) are important targets for drugs and pesticides. The macrocyclic lactone abamectin primarily targets GluCl and is commonly used to control the spider mite Tetranychus urticae, an economically important crop pest. However, abamectin resistance has been reported for multiple T. urticae populations worldwide, and in several cases was associated with the mutations G314D in GluCl1 and G326E in GluCl3. Recently, an additional I321T mutation in GluCl3 was identified in several abamectin resistant T. urticae field populations. Here, we aim to functionally validate this mutation and determine its phenotypic strength. RESULTS The GluCl3 I321T mutation was introgressed into a T. urticae susceptible background by marker‐assisted backcrossing, revealing contrasting results in phenotypic strength, ranging from almost none to 50‐fold. Next, we used CRISPR‐Cas9 to introduce I321T, G314D and G326E in the orthologous Drosophila GluCl. Genome modified flies expressing GluCl I321T were threefold less susceptible to abamectin, while CRISPRed GluCl G314D and G326E flies were lethal. Last, functional analysis in Xenopus oocytes revealed that the I321T mutation might reduce GluCl3 sensitivity to abamectin, but also suggested that all three T. urticae Rdls are affected by abamectin. CONCLUSION Three different techniques were used to characterize the role of I321T in GluCl3 in abamectin resistance and, combining all results, our analysis suggests that the I321T mutation has a complex role in abamectin resistance. Given the reported subtle effect, additional synergistic factors in resistance warrant more investigation. © 2020 Society of Chemical Industry

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Mite Strainsmentioning

confidence: 99%

See 2 more Smart Citations

Untangling a Gordian knot: the role of a GluCl3 I321T mutation in abamectin resistance in Tetranychus urticae

Xue

Mermans

Papapostolou

et al. 2020

Pest Management Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…The T. urticae Koch is a widespread polyphagous pest that attacks more than 1100 different plant species [9,11], making it one of the main phytosanitary problems for many crops. This trait is owing (among other reasons) to its capacity for quickly generating resistance to synthetic acaricidal products [12]—from two to four years of new active ingredients [9]—even after a few applications of the active ingredient [11].…”

Section: Characteristics Of T Urticaementioning

confidence: 99%

Botanicals Against Tetranychus urticae Koch Under Laboratory Conditions: A Survey of Alternatives for Controlling Pest Mites

Rincón

Rodríguez

Coy-Barrera

2019

Plants

View full text Add to dashboard Cite

Tetranychus urticae Koch is a phytophagous mite capable of altering the physiological processes of plants, causing damages estimated at USD$ 4500 per hectare, corresponding to approximately 30% of the total cost of pesticides used in some important crops. Several tools are used in the management of this pest, with chemical control being the most frequently exploited. Nevertheless, the use of chemically synthesized acaricides brings a number of disadvantages, such as the development of resistance by the pest, hormolygosis, incompatibility with natural predators, phytotoxicity, environmental pollution, and risks to human health. In that sense, the continuous search for botanical pesticides arises as a complementary alternative in the control of T. urticae Koch. Although a lot of information is unknown about its mechanisms of action and composition, there are multiple experiments in lab conditions that have been performed to determine the toxic effects of botanicals on this mite. Among the most studied botanical families for this purpose are plants from the Lamiaceae, the Asteraceae, the Myrtaceae, and the Apiaceae taxons. These are particularly abundant and exhibit several results at different levels; therefore, many of them can be considered as promising elements to be included into integrated pest management for controlling T. urticae.

show abstract

Geographical distribution and molecular insights into abamectin and milbemectin cross‐resistance in European field populations of Tetranychus urticae

Xue

Snoeck

Njiru

et al. 2020

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Milbemectin and abamectin are frequently used to control the spider 35 mite Tetranychus urticae. The development of abamectin resistance in this major pest 36 has become an increasing problem worldwide, potentially compromising the use of 37 milbemectin. In this study, a large collection of European field populations was 38 screened for milbemectin and abamectin resistance, allowing to thoroughly evaluate 39 any potential cross-resistance risk, and both target-site and metabolic resistance 40 mechanisms were investigated.41 RESULTS: High to very high levels of abamectin resistance were found in one third of 42 all populations, while milbemectin resistance levels were low for most populations. The 43 occurrence of well-known target-site resistance mutations in glutamate-gated chloride 44 channels (G314D in GluCl1 and G326E in GluCl3) was documented in the most 45 resistant populations. However, a new mutation, I321T in GluCl3, was also uncovered 46 in three resistant populations. A differential gene-expression analysis revealed the 47 overexpression of detoxification genes, more specifically cytochrome P450 48 monooxygenase (P450) and UDP-glycosyltransferase (UGT) genes. Subsequently, 49 multiple UGTs were functionally expressed, and their capability to glycosylate 50 abamectin and milbemectin, was tested and confirmed. 51 CONCLUSIONS: We found a clear correlation between abamectin and milbemectin 52 resistance in European populations of T. urticae, but as milbemectin resistance levels 53 were low, the observed cross-resistance is probably not of operational importance. The 54 presence of target-site resistance mutations in GluCl genes was confirmed in most but 55 not all resistant populations. Gene-expression analysis andr functional characterization 56 of P450s and UGTs suggests that also metabolic abamectin resistance mechanisms 57 are common in European T. urticae populations.

show abstract

Resistance incidence and presence of resistance mutations in populations of Tetranychus urticae from vegetable crops in Turkey

Cited by 38 publications

References 65 publications

Untangling a Gordian knot: the role of a GluCl3 I321T mutation in abamectin resistance in Tetranychus urticae

Untangling a Gordian knot: the role of a GluCl3 I321T mutation in abamectin resistance in Tetranychus urticae

Botanicals Against Tetranychus urticae Koch Under Laboratory Conditions: A Survey of Alternatives for Controlling Pest Mites

Geographical distribution and molecular insights into abamectin and milbemectin cross‐resistance in European field populations of Tetranychus urticae

Contact Info

Product

Resources

About